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Comment
. 2018 Dec 13;14(12):e1007809.
doi: 10.1371/journal.pgen.1007809. eCollection 2018 Dec.

R-loops modulate Trypanosome antigenic variation

Lauren L Prister  1 , H Steven Seifert  1
Affiliations
Comment

R-loops modulate Trypanosome antigenic variation

Lauren L Prister et al. PLoS Genet. .
No abstract available

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. R-loop levels at silent and active ES.
Briggs and colleagues performed DRIP-Seq and mapped the results to the active ES, VSG221 (Purple), and all silent ES loci (green). There are also expression site–associated genes located in the ES but are not pictured. R-loop levels were detected in WT cells and TbRH1 −/− cells (blue and orange, respectively). Below the loci, the relative R-loop levels for each region are shown; taller boxes represent increased detection of R-loops. 5′ to the 70-bp repeats R-loops were increased in the TbRH1 mutant (vertical stripes). When TbRH1 is absent, R-loops were increased over the 70-bp repeats in both the active and silent repeats (orange waves and stripes). 3′ of the VSGs in both silent and active loci, R-loops were increased, which are suggested to represent telomeric R-loops (orange checkered box). DRIP-Seq, DNA:RNA hybrid immunoprecipitation; ES, expressed copy; TbRH1, RNaseH1 enzyme of T. brucei; VSG, variable surface glycoprotein; WT, wild-type.
See this image and copyright information in PMC

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References

    1. Vink C, Rudenko G, Seifert HS. Microbial antigenic variation mediated by homologous DNA recombination. FEMS microbiology reviews. 2012;36(5):917–48. 10.1111/j.1574-6976.2011.00321.x ; PubMed Central PMCID: PMC3334452. - DOI - PMC - PubMed
    1. Aitcheson N, Talbot S, Shapiro J, Hughes K, Adkin C, Butt T, et al. VSG switching in Trypanosoma brucei: antigenic variation analysed using RNAi in the absence of immune selection. Molecular microbiology. 2005;57(6):1608–22. Epub 2005/09/02. MMI4795 [pii] 10.1111/j.1365-2958.2005.04795.x ; PubMed Central PMCID: PMC1618954. - DOI - PMC - PubMed
    1. Boothroyd CE, Dreesen O, Leonova T, Ly KI, Figueiredo LM, Cross GA, et al. A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Nature. 2009;459(7244):278–81. Epub 2009/04/17. nature07982 [pii] 10.1038/nature07982 ; PubMed Central PMCID: PMC2688456. - DOI - PMC - PubMed
    1. Briggs E, Crouch K., Lemgruber, Lapsey, McCulloch R. Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion. PLoS Genet. 2018;14(12). doi: e1007729 - PMC - PubMed
    1. McCulloch R, Morrison LJ, Hall JPJ. DNA Recombination Strategies During Antigenic Variation in the African Trypanosome. Microbiol Spectr. 2015;3(2):MDNA3-0016-2014. 10.1128/microbiolspec.MDNA3-0016-2014 . - DOI - PubMed