Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Abstract

Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress. Results and conclusion: RTCA is attracting more and more attention. Now it plays an important role in drug screening, toxicology, Chinese herbal medicine and so on. It has wide application prospects in the area of modern pharmaceutical evaluation and analysis.

Keywords: RTCA; application progress; pharmaceutical evaluation; traditional Chinese medicine.

Figure 1

Schematic diagram for the mechanism of Real-Time Cell-based Assay.

Figure 2

CI-t curve of MCF-7 cell line based on different concentration of Panax notoginseng extractive (curve from up to bottom show the concentration as con, 0.17, 0.28, 0.47, 0.78, 1.3, 2.16, 3.6 mg/mL).

Figure 3

Effect of different sample concentrations on real-time cell analyzer curves generated with LX2 cells. Effect of 2 mg/mL (red line), 1.5 mg/mL (green line), 1.0 mg/mL (blue line), 0.78 mg/mL (pink line), 0.39 mg/mL (turquoise line), 0.19 mg/mL (purple line), CON (brown line, control group without treatment).