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. 2018 Dec 12;23(12):3296.
doi: 10.3390/molecules23123296.

Oleiferasaponin A₂, a Novel Saponin from Camellia oleifera Abel. Seeds, Inhibits Lipid Accumulation of HepG2 Cells Through Regulating Fatty Acid Metabolism

Tai-Mei Di  1 , Shao-Lan Yang  2 , Feng-Yu Du  3 , Lei Zhao  4 , Xiao-Han Li  5 , Tao Xia  6 , Xin-Fu Zhang  7
Affiliations

Oleiferasaponin A₂, a Novel Saponin from Camellia oleifera Abel. Seeds, Inhibits Lipid Accumulation of HepG2 Cells Through Regulating Fatty Acid Metabolism

Tai-Mei Di et al. Molecules. .

Abstract

A new triterpenoid saponin, named oleiferasaponin A₂, was isolated and identified from Camellia oleifera defatted seeds. Oleiferasaponin A₂ exhibited anti-hyperlipidemic activity on HepG2 cell lines. Further study of the hypolipidemic mechanism showed that oleiferasaponin A₂ inhibited fatty acid synthesis by significantly down-regulating the expression of SREBP-1c, FAS and FAS protein, while dramatically promoting fatty acid β-oxidation by up-regulating the expression of ACOX-1, CPT-1 and ACOX-1 protein. Our results demonstrate that the oleiferasaponin A₂ possesses potential medicinal value for hyperlipidemia treatment.

Keywords: Camellia oleifera; fatty acid metabolism; hypolipidemic activity; oleiferasaponin A2; triterpenoid saponin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
(A) Structure of oleiferasaponin A2; and (B) Key HMBC correlations of oleiferasaponin A2.
Figure 2
Figure 2
(A) The effect of oleiferasaponin A2 on HepG2 cells proliferation; (B) the lipid lowering activity of oleiferasaponin A2 under oleic acid treatment; and (C) the content of triglyceride under OA inducement. NC, blank control without oleic acid; 0, model group with 80 μM oleic acid; SVST, group with 80 μM oleic acid and 10 μM simvastain; A2, group with 80 μM oleic acid and 10 μM oleiferasaponin A2. ** p < 0.01, * p < 0.05.
Figure 3
Figure 3
The relative expression of genes related to fatty acid metabolism on fatty acid synthesis genes (SREBP-1c, FAS and ACC) and fatty acid β-oxidation genes (PPARα, ACOX-1 and CPT-1). NC, blank control; OA, 80 μM oleic Acid; OA + SVST, 80 μM Oleic Acid + 10 μM simvastatin; OA + A2, 80 μM Oleic Acid + 10 μM Oleiferasapoin A2. Compared to the control, ** p < 0.01.
Figure 4
Figure 4
The relative expression of proteins related to fatty acid metabolism. NC, blank control without oleic acid; 0, model group with 80 μM oleic acid; SVST, group with 80 μM oleic acid and 10 μM simvastain; A2, group with 80 μM oleic acid and 10 μM oleiferasapoin A2; ACOX-1, fatty acid oxidation protein; FAS, fatty acid synthesis protein; β-actin, reference protein.
See this image and copyright information in PMC

References

    1. Melanie N., Townsend N., Scarnorough P., Rayner M. Cardiovascular disease in Europe 2014: Epidemiological update. Eur. Heart J. 2014;35:2950–2959. - PubMed
    1. Program L.C. The lipid research clinics coronary primary prevention trial results. I. Reduction in incidence of coronary heart disease. JAMA. 1985;253:635–636. - PubMed
    1. Shrestha S., Bhattarai B.R., Lee K.H., Cho H. Mono- and disalicylic acid derivatives: Ptp1b inhibitors as potential anti-obesity drugs. Bioorgan. Med. Chem. 2007;15:6535–6548. doi: 10.1016/j.bmc.2007.07.010. - DOI - PubMed
    1. Sharma S.B., Nasir A., Prabhu K.M., Murthy P.S., Dev G. Hypoglycemic and hypolipidemic effect of ethanolic extract of seeds of eugenia jambolana in alloxan induced diabetic rabbits. J. Ethnopharmacol. 2003;85:201–206. doi: 10.1016/S0378-8741(02)00366-5. - DOI - PubMed
    1. Zou B., Li C.M., Chen J.Y., Dong X.Q., Zhang Y., Du J. High molecular weight persimmon tannin is a potent hypolipidemic in high-cholesterol diet fed rats. Food Res. Int. 2012;48:970–977. doi: 10.1016/j.foodres.2012.05.024. - DOI