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. 2018 Dec;16(6):6966-6970.
doi: 10.3892/ol.2018.9554. Epub 2018 Oct 5.

Relationship between the genetic expression of WTAP and bladder cancer and patient prognosis

Lezhong Chen  1   2 , Xinghuan Wang  1
Affiliations

Relationship between the genetic expression of WTAP and bladder cancer and patient prognosis

Lezhong Chen et al. Oncol Lett. 2018 Dec.

Abstract

The relationship between the gene expression of Wilms tumor 1-associated protein (WTAP) and bladder cancer was investigated to study the effect of its expression on patient prognosis. Sixty-two fresh specimens of bladder transitional cell cancer tissues were collected as the bladder cancer group, while 20 normal bladder mucosa specimens comprised the control group. Hematoxylin and eosin staining was conducted to detect the pathological differences between the groups and the immunohistochemistry was used to test the expression levels of WTAP in the tissues. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of WTAP. Moreover, western blot analysis was used to examine the WTAP expression levels. At the same time, Cox regression multi-factor survival analysis was conducted for the related factors to the prognoses of bladder cancer patients. The structures of cells in the bladder cancer group were destroyed as was evident by the shrunken cell nuclei, while the tissues in the control group were intact. WTAP expression in the bladder cancer group was significantly increased compared with that in the control group. A small number of mRNAs and proteins were higher in the bladder cancer group than that in the control group. The differences in WTAP expression between the bladder cancer and control groups were statistically significant (P<0.05). There were obvious differences in the postoperative recurrence risk between the patients with a negative WTAP protein expression and those with a positive one (P<0.05). In conclusion, WTAP may play an important role in the occurrence and development of bladder cancer and can be considered as a potential target for bladder cancer treatment, providing a new basis for clinical diagnoses.

Keywords: WTAP; bladder cancer; relationship with prognosis.

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Figures

Figure 1.
Figure 1.
Hematoxylin and eosin (H&E) staining results of tissues of the control group and the bladder cancer group (×200). Compared with tissues of the control group, those of the bladder cancer group are destroyed and the cell nuclei are damaged; moreover, a large number of inflammatory infiltrating cells with the significant histopathology is observed.
Figure 2.
Figure 2.
Immunohistochemical staining results of Wilms tumor 1-associated protein (WTAP) in tissues of the control group and the bladder cancer group (×200). WTAPs are expressed lowly in the control group, while they are expressed at a high level in the bladder cancer group.
Figure 3.
Figure 3.
RT-PCR results of Wilms tumor 1-associated protein (WTAP) mRNAs in the control group and the bladder cancer group. P<0.05 compared with the control group.
Figure 4.
Figure 4.
Western blot results of Wilms tumor 1-associated proteins (WTAPs) in the control group and the bladder cancer group. **P<0.01 compared with the control group.
Figure 5.
Figure 5.
The relationship between Wilms tumor 1-associated protein (WTAP) and the prognosis of bladder cancer patients. The total survival period in the negative WTAP expression group is significantly higher than that in the positive WTAP expression group.
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