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. 2019 Jan;33(1):124-131.
doi: 10.1111/jvim.15376. Epub 2018 Dec 11.

Influence of canine donor plasma hemostatic protein concentration on quality of cryoprecipitate

Macy Drinkhouse  1 , Marjory B Brooks  2 , Darko Stefanovski  3 , Kimberly Marryott  1 , Mary Beth Callan  1
Affiliations

Influence of canine donor plasma hemostatic protein concentration on quality of cryoprecipitate

Macy Drinkhouse et al. J Vet Intern Med. 2019 Jan.

Abstract

Background: Cryoprecipitate (CRYO) is a plasma component containing high concentrations of factor VIII (FVIII), von Willebrand factor (VWF), and fibrinogen. Because Greyhounds are reported to have lower plasma VWF and fibrinogen concentrations, their plasma may not yield high potency CRYO.

Objectives: To determine if plasma hemostatic protein concentration is a good predictor of CRYO potency and if a difference exists in quality of CRYO prepared from Greyhounds versus non-Greyhounds.

Animals: Twenty Greyhounds and 20 non-Greyhounds.

Methods: A 450 mL unit of blood was collected from each donor, centrifuged to prepare fresh frozen plasma (FFP), and processed to CRYO. Aliquots of FFP and CRYO were analyzed for FVIII, VWF, and fibrinogen content and factor recovery.

Results: A positive correlation was found among donor plasma FVIII, VWF and fibrinogen concentration, and CRYO factor content (P < .001). Mean recovery was highest for VWF (67%), followed by fibrinogen (47%), and FVIII (37%). No breed difference was found in mean CRYO FVIII content, but CRYO VWF and fibrinogen were lower in Greyhounds (P = .004 and P < .001, respectively). No difference was found between Greyhounds and non-Greyhounds for the number of CRYO units meeting human blood banking standards.

Conclusions and clinical importance: Factor concentration in FFP is associated with CRYO potency, suggesting that prescreening of blood donors may enhance CRYO quality. Despite lower VWF and fibrinogen content, CRYO prepared from Greyhounds is acceptable based on blood banking standards for humans, indicating that Greyhound FFP does not need to be excluded from CRYO production.

Keywords: Greyhound; Von Willebrand factor; factor VIII; fibrinogen.

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Figures

Figure 1
Figure 1
Histograms of distribution of cryoprecipitate (CRYO) hemostatic protein content for: A, von Willebrand factor (VWF); B, factor VIII (FVIII); and C, fibrinogen. Open bars represent Greyhounds (n = 20), and filled bars represent non‐Greyhounds (n = 20). The width of each bar represents 200 U/unit for VWF, 100 U/unit for FVIII, and 100 mg/unit for fibrinogen
Figure 2
Figure 2
Spearman correlation between canine donor plasma (FFP) hemostatic protein concentration and cryoprecipitate (CRYO) hemostatic protein content for: A, von Willebrand factor (VWF); B, factor VIII (FVIII); and C, fibrinogen
See this image and copyright information in PMC

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