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. 2019 Apr;17(4):709-711.
doi: 10.1111/pbi.13053. Epub 2019 Jan 2.

xCas9 expands the scope of genome editing with reduced efficiency in rice

Junjie Wang  1 , Xiangbing Meng  2 , Xixun Hu  1 , Tingting Sun  1 , Jiayang Li  2   3 , Kejian Wang  1 , Hong Yu  2
Affiliations

xCas9 expands the scope of genome editing with reduced efficiency in rice

Junjie Wang et al. Plant Biotechnol J. 2019 Apr.
No abstract available

Keywords: genome editing; rice; xCas9.

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Figures

Figure 1
Figure 1
Genome Editing in Rice Using xCas9 3.6 and 3.7 Variants. (a) Schematic illustration of the generation of xCas9 3.6 and 3.7 variants. NLSs: nuclear localization signals, CaMV T: cauliflower mosaic virus 35S terminator, OsActin P: OsActin promoter. Red lines represent differences in amino acid positions compared with SpCas9. (b) Efficiency of genome editing using reportedly efficient protospacer adjacent motifs in three different endogenous rice genes. Error bars represent mean ± SEM (n = 3 independent replicates, with each independent replicate containing 16 transformed calli). (c) Efficiency of genome editing at CAA and NNG PAM sites in three different endogenous rice genes. Error bars represent mean ± SEM (n = 3 independent replicates, with each independent replicate containing 16 transformed calli). (d) Efficiency of genome editing at NAG PAM sites in three different endogenous rice genes. Error bars represent mean ± SEM (n = 3 independent replicates, with each independent replicate containing 16 transformed calli). (e) Summary of edited calli as tested by Hi‐TOM analysis. Hi‐TOM analysis filter threshold is set as >5%. Total number of detected calli is 48, (three independent replicates, with each independent replicate containing 16 transformed calli). MOC1: MONOCULM1, D14: DWARF14 and PDS: PHYTOENE DESATURASE [Colour figure can be viewed at wileyonlinelibrary.com]
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References

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