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. 2019 Jan;9(1):1.
doi: 10.1007/s13205-018-1515-5. Epub 2018 Dec 5.

Characterization of a new exopolysaccharide produced by Halorubrum sp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells

Masoud Hamidi  1 , Rasool Mirzaei  1 , Cédric Delattre  2 , Korosh Khanaki  1 , Guillaume Pierre  2 , Christine Gardarin  2 , Emmanuel Petit  3 , Fatemeh Karimitabar  1 , Sobhan Faezi  1
Affiliations

Characterization of a new exopolysaccharide produced by Halorubrum sp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells

Masoud Hamidi et al. 3 Biotech. 2019 Jan.

Abstract

In the present study, we aimed to extract, purify, analyze monosaccharide composition of exopolysaccharide (EPS) produced by Halorubrum sp. TBZ112 (KCTC 4203 and IBRC-M 10773) and also to evaluate its possible antiproliferative activity against human gastric cancer (MKN-45) cell line and its biocompatibility effect on normal cells using human dermal fibroblast (HDF) cell line. Average molecular weight and monosaccharide composition were determined by high-pressure size exclusion chromatography (HPSEC) with multi-angle laser light scattering (MALLS) and high-pressure anion exchange chromatography (HPAEC), respectively. Fourier transform infrared (FTIR) spectroscopy was used for the partial characterization of the EPS. The EPS effect on the cell proliferation and viability of MKN-45 and HDF cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue dye exclusion, respectively. Strain TBZ112 excreted 480 mg.l-1 of the EPS under optimal growth conditions. The EPS had a molecular weight of 5.052 kDa and was a heteropolysaccharide containing ten moieties mainly composed of mannose (19.95%), glucosamine (15.55%), galacturonic acid (15.43%), arabinose (12.24%), and glucuronic acid (12.05%). No significant difference of the EPS treatments on the proliferation activity of MKN-45 and HDF cells were observed (P > 0.05). For the first time, the EPS from Halorubrum sp. TBZ112, an extremely halophilic archaeon related to Halorubrum genus, was isolated and chemically characterized. The EPS from Halorubrum sp. TBZ112 possesses a relatively low molecular weight and might be applied as a biocompatible compound. More investigations are needed to determine other biological activities of the EPS along with further details of its chemical structure.

Keywords: Antiproliferative effect; Exopolysaccharide (EPS); Halorubrum sp. TBZ112; Monosaccharide composition.

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Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
FTIR spectrum of the EPS isolated from Halorubrum sp.TBZ112 (KCTC 4203 and IBRC-M 10773). The band at 3284.72 cm−1 might be attributed to the stretching of hydroxyl group as well as water adsorption. The band at 1635 cm−1 was a result of the C=O stretching. The ether group from the EPS was indicated at 1082.09 cm−1 absorption band. The absence of symmetric strong band in the frequency of 1150 cm−1 and asymmetric strong band in the frequency of 1200 cm−1 (Pavia et al. 2008) demonstrated that the EPS from Halorubrum sp. TBZ112 most likely did not include S=O group
Fig. 2
Fig. 2
Proliferation (survival) activity in human gastric cancer (MKN-45) cell line (a) and human dermal fibroblast (HDF) cell line (b) under exopolysaccharide treatments after 24 and 48 h. Data are presented as mean ± SD
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