Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

Abstract

: More than 50 papers on surface plasmon resonance biosensors, published between 2016 and mid-2018, are reviewed. Papers concerning the determination of large particles such as vesicles, exosomes, cancer cells, living cells, stem cells, and microRNA are excluded, as these are covered by a very recent review. The reviewed papers are categorized into five groups, depending on the degree of maturity of the reported solution; ranging from simple marker detection to clinical application of a previously developed biosensor. Instrumental solutions and details of biosensor construction are analyzed, including the chips, receptors, and linkers used, as well as calibration strategies. Biosensors with a sandwich structure containing different nanoparticles are considered separately, as are SPR (Surface Plasmon Resonance) applications for investigating the interactions of biomolecules. An analysis is also made of the markers determined using the biosensors. In conclusion, there is shown to be a growing number of SPR applications in the solution of real clinical problems.

Keywords: antibodies; biosensors; cancer markers; nanoparticles; receptor immobilization; surface plasmon resonance.

Figure 1

Example of fluidic SPR measurement. Reproduced with permission from [4]. Copyright (2018) Elsevier BV.

Figure 2

An array of gold nanohole measuring points used in microfluidic measurement. Reproduced with permission from [3]. Copyright 2015, Springer Science Business Media New York.

Figure 3

Example of non-fluidic measurement. Reproduced with permission from [27]. Copyright 2017 Elsevier B.V.

Figure 4

Example of covalent antibody immobilization (c) and picture of the chip (a) and image of the chip obtained by a CCD camera (b). Reproduced from [22] with permission of Royal Society of Chemistry.

Figure 5

Example of receptor immobilization via hydrophobic interaction. Reproduced from [24] with permission of Royal Society of Chemistry.

Figure 6

Example of the use of nanoparticles for SPR signal enhancement. (a) Scheme of nanparticles processing, (b) sandwich assay. Reproduced with permission from [38]. Copyright 2017, Springer-Verlag Berlin Heidelberg.

Figure 7

Typical calibration curves.