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. 2019 Jan;25(1):166-170.
doi: 10.3201/eid2501.180267.

Isolation and Full-Genome Characterization of Nipah Viruses from Bats, Bangladesh

Danielle E Anderson, Ariful Islam, Gary Crameri, Shawn Todd, Ausraful Islam, Salah U Khan, Adam Foord, Mohammed Z Rahman, Ian H Mendenhall, Stephen P Luby, Emily S Gurley, Peter Daszak, Jonathan H Epstein, Lin-Fa Wang

Isolation and Full-Genome Characterization of Nipah Viruses from Bats, Bangladesh

Danielle E Anderson et al. Emerg Infect Dis. 2019 Jan.

Abstract

Despite molecular and serologic evidence of Nipah virus in bats from various locations, attempts to isolate live virus have been largely unsuccessful. We report isolation and full-genome characterization of 10 Nipah virus isolates from Pteropus medius bats sampled in Bangladesh during 2013 and 2014.

Keywords: Bangladesh; Nipah virus; Pteropus medius; bats; enrichment; full-genome characterization; henipavirus; isolation; next-generation sequencing; viruses; zoonoses.

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Figures

Figure 1
Figure 1
Sampling sites (Raipur, Manikganj, and Sylhet) for bat reservoirs of Nipah virus and location of the capital, Dhaka, in Bangladesh. Map was generated by using ArcGIS version 10.4.1 software (https://desktop.arcgis.com/en/quick-start-guides/10.4/arcgis-desktop-quick-start-guide.htm).
Figure 2
Figure 2
Phylogenetic tree of Nipah viruses from bats in Bangladesh (bold) compared with other henipaviruses, generated from full-genome sequences. Tree was constructed by using a maximum-likelihood approach, and robustness of nodes was tested with 1,000 bootstrap replicates. Sequences are labeled according to the following ordination: GenBank accession number or isolate identification number/virus type/country/host/year/strain. Numbers along branches are bootstrap values. Scale bar indicates nucleotide substitutions per site.
See this image and copyright information in PMC

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