Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2019 Jan 2;116(1):20-22.
doi: 10.1073/pnas.1819614116. Epub 2018 Dec 18.

Probing mitochondrial metabolism in vivo

Gregory S McElroy  1 , Navdeep S Chandel  2
Affiliations
Comment

Probing mitochondrial metabolism in vivo

Gregory S McElroy et al. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement: N.S.C. has collaborated with David M. Sabatini and Kivanç Birsoy.

Figures

Fig. 1.
Fig. 1.
MITO-Tag Mice use Cre-Lox recombination to express a mitochondria-targeted epitope tag in specific cell types or tissues. This epitope tag allows the rapid and direct isolation of mitochondria from the cell types or tissues of interest. Mitochondria isolated from these tissues can then be analyzed using multiomic approaches, including metabolite and protein identification and quantification by mass spectrometry.
See this image and copyright information in PMC

Comment on

References

    1. Mehta MM, Weinberg SE, Chandel NS. Mitochondrial control of immunity: Beyond ATP. Nat Rev Immunol. 2017;17:608–620. - PubMed
    1. Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Absolute quantification of matrix metabolites reveals the dynamics of mitochondrial metabolism. Cell. 2016;166:1324–1337.e11. - PMC - PubMed
    1. Bayraktar EC, et al. MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo. Proc Natl Acad Sci USA. 2019;116:303–312. - PMC - PubMed
    1. Chen WW, Freinkman E, Sabatini DM. Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites. Nat Protoc. 2017;12:2215–2231. - PMC - PubMed
    1. Van Vranken JG, Rutter J. The whole (cell) is less than the sum of its parts. Cell. 2016;166:1078–1079. - PMC - PubMed

LinkOut - more resources