Epidermal growth factor and insulin-like growth factor I stimulate the hydrolysis of the insulin-sensitive phosphatidylinositol-glycan in BC3H-1 myocytes

Abstract

BC3H-1 myocytes contain a phospholipid(s) which is labeled with [3H]inositol, [3H]glucosamine, and [3H]myristate [a phosphatidylinositol-glycan (PI-glycan)], and which is hydrolyzed by insulin induced activation of a specific phospholipase C. Similarly, epidermal growth factor and insulin-like growth factor-I provoke rapid increases in the hydrolysis of this PI-glycan, suggesting that derived signaling substances may be important in the action of agonists which activate tyrosine kinase type receptors.