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. 2018 Dec 4:9:602.
doi: 10.3389/fgene.2018.00602. eCollection 2018.

Ethanol's Effect on Coq7 Expression in the Hippocampus of Mice

Diana Zhou  1 , Yinghong Zhao  2 , Michael Hook  1 , Wenyuan Zhao  1 , Athena Starlard-Davenport  1 , Melloni N Cook  1   3 , Byron C Jones  1 , Kristin M Hamre  4 , Lu Lu  1
Affiliations

Ethanol's Effect on Coq7 Expression in the Hippocampus of Mice

Diana Zhou et al. Front Genet. .

Abstract

Coenzyme Q (CoQ) is a well-studied molecule, present in every cell membrane in the body, best known for its roles as a mitochondrial electron transporter and a potent membrane anti-oxidant. Much of the previous work was done in vitro in yeast and more recent work has suggested that CoQ may have additional roles prompting calls for a re-assessment of its role using in vivo systems in mammals. Here we investigated the putative role of Coenzyme Q in ethanol-induced effects in vivo using BXD RI mice. We examined hippocampal expression of Coq7 in saline controls and after an acute ethanol treatment, noting enriched biologic processes and pathways following ethanol administration. We also identified 45 ethanol-related phenotypes that were significantly correlated with Coq7 expression, including six phenotypes related to conditioned taste aversion and ethanol preference. This analysis highlights the need for further investigation of Coq7 and related genes in vivo as well as previously unrecognized roles that it may play in the hippocampus.

Keywords: Coenzyme Q; ethanol; genetics; genomics; hippocampus; mouse models; oxidative stress.

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Figures

FIGURE 1
FIGURE 1
Differential expression of Coq7 across BXD RI strains rank ordered by expression level. The standard deviation and mean expression of Coq7 in each strain is shown across the parental DBA/2J and C57BL/6J strains, F1 hybrids, and 67 BXD strains. The x-axis represents the mouse strain, while the y-axis shows the mean gene expression using the log2 scale.
FIGURE 2
FIGURE 2
GEMMA eQTL mapping for Coq7 expression in hippocampus. The x-axis represents positions on the mouse genome in megabases (Mb), while the y-axis represents the likelihood ratio statistic scores (LRS), a measurement of the linkage between expression of Coq7 and inheritance of B6 and D2 alleles at different regions of the genome. Top panel shows interval mapping for the whole mouse genome, indicating a significant eQTL at chromosome 7. Bottom panel shows a close-up view of chromosome 7 in which the peak of eQTL was around 119 Mb of chromosome 7 where Coq7 is located (triangle).
FIGURE 3
FIGURE 3
GEMMA QTL mapping for locomotion behavior after ethanol injection (2 g/kg). The x-axis represents a position on the mouse genome in megabases (Mb), while the y-axis gives the likelihood ratio statistic scores (LRS). The mapping result showed that the peak of QTL was around 114∼119 Mb of chromosome 7.
See this image and copyright information in PMC

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