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. 2018 Nov 14;5(11):180551.
doi: 10.1098/rsos.180551. eCollection 2018 Nov.

Microbial oil production from solid-state fermentation by a newly isolated oleaginous fungus, Mucor circinelloides Q531 from mulberry branches

Weichuan Qiao  1 , Junqi Tao  1 , Yang Luo  1 , Tianhao Tang  1 , Jiahui Miao  1 , Qiwen Yang  1
Affiliations

Microbial oil production from solid-state fermentation by a newly isolated oleaginous fungus, Mucor circinelloides Q531 from mulberry branches

Weichuan Qiao et al. R Soc Open Sci. .

Abstract

In this study, a newly isolated oleaginous fungus, Mucor circinelloides (M. circinelloides) Q531, was able to convert mulberry branches into lipids. The highest yield and the maximum lipid content produced by the fungal cells were 42.43 ± 4.01 mg per gram dry substrate (gds) and 28.8 ± 2.85%, respectively. The main components of lignocellulosic biomass were gradually reduced during solid-state fermentation (SSF). Cellulose, hemicellulose and lignin were decreased from 45.11, 31.39 and 17.36% to 41.48, 28.71, and 15.1%, respectively. Gas chromatography analysis showed that the major compositions of the fermented products were palmitic acid (C16:0, 18.42%), palmitoleic acid (C16:1, 5.56%), stearic acid (C18:0, 5.87%), oleic acid (C18:1, 33.89%), linoleic acid (C18:2, 14.45%) and γ-linolenic acid (C18:3 n6, 22.53%) after 2 days of SSF. The fatty acid methyl esters contained unsaturated fatty acids with a ratio of 75.95%. The composition and content obtained in this study are more advantageous than those of many other biomass lipids. Meanwhile, the oleaginous fungus had a high cellulase activity of 1.39 ± 0.09 FPU gds-1. The results indicate that the enzyme activity of the isolated fungus was capable of converting the cellulose and hemicelluloses to available sugar monomers which are beneficial for the production of lipids.

Keywords: lignocellulose; microbial lipid; mulberry branches; oleaginous fungi; solid-state fermentation.

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Conflict of interest statement

We have no competing interests.

Figures

Figure 1.
Figure 1.
Neighbour-joining phylogenetic tree based on the 18S rDNA sequences shows the relationship between the newly isolated M. circinelloides Q531 and other members of the genus Mucor. The number of replicates is 1000. Numbers following the names of the strains are accession numbers of published sequences in the GenBank database.
Figure 2.
Figure 2.
Photographs of lipid globules in the newly isolated M. circinelloides Q531 undergoing SSF taken by a microscope with a ×1000 oil immersion lens.
Figure 3.
Figure 3.
Lipid yield, biomass and cellulase activity of M. circinelloides Q531 in solid-state fermentation medium over time.
Figure 4.
Figure 4.
Residual sugars produced by M. circinelloides Q531 in solid-state fermentation medium over time.
Figure 5.
Figure 5.
The change in lignocellulosic biomass (cellulose, hemicellulose and lignin) composition in solid-state fermentation over time.
Figure 6.
Figure 6.
The composition of major fatty acids of lipids produced by M. circinelloides Q531 in solid-state fermentation over time.
See this image and copyright information in PMC

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