Genotyping methods and molecular epidemiology of Mycobacterium avium subsp. paratuberculosis (MAP)

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease (JD) which affects mainly ruminants and is characterized by chronic diarrhea and emaciation. Johne's disease is highly prevalent in many countries around the world and leads to high economic losses associated with decreased production. Genotyping of the involved pathogen could be used in the study of population genetics, pathogenesis and molecular epidemiology including disease surveillance and outbreak investigation. Principally, researchers have first assumed the presence of two different MAP strains that are associated with the animal host species (cattle and sheep). However, nowadays MAP characterization depends mainly upon genetic testing using genetic markers such as insertion elements, repetitive sequences and single nucleotide polymorphisms. This work aims to provide an overview of the advances in molecular biological tools used for MAP typing in the last two decades, discuss how these methods have been used to address interesting epidemiological questions, and explore the future prospects of MAP molecular epidemiology given the ever decreasing costs of the high throughput sequencing technology.

Keywords: Genetic markers; Johne’s disease; Molecular epidemiology; Paratuberculosis; Sequencing; Single nucleotide polymorphisms.

Fig. 1

Nomenclature and classification of MAP types based on a recent whole genome sequencing-based phylogenetic analysis . Type C (cattle type), Type S (sheep type) and type B (bison type).