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. 1988 Nov 21;240(1-2):115-7.
doi: 10.1016/0014-5793(88)80350-8.

Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression

R Yamaguchi  1 , K Matsuo, A Yamazaki, S Nagai, K Terasaka, T Yamada
Affiliations
Free article

Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression

R Yamaguchi et al. FEBS Lett. .
Free article

Abstract

Using a single-probe method, we have cloned the gene for an immunogenic MPB57 protein of Mycobacterium bovis BCG. The nucleotide sequence includes an ORF of 300 base pairs encoding a protein of 99 amino acids with an Mr of 10,818. This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57.

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