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. 2018 Dec 5:12:105.
doi: 10.3389/fnana.2018.00105. eCollection 2018.

Three-Dimensional Nanostructure of an Intact Microglia Cell

Giulia Bolasco  1 , Laetitia Weinhard  1 , Tom Boissonnet  1 , Ralph Neujahr  2 , Cornelius T Gross  1
Affiliations

Three-Dimensional Nanostructure of an Intact Microglia Cell

Giulia Bolasco et al. Front Neuroanat. .
No abstract available

Keywords: CLEM; SBEM; TEM; microglia; segmentation; ultrastructure; volumetric.

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Figures

Figure 1
Figure 1
Correlative light and electron microscopy workflow. Confocal imaging of microglia (tdTomato, red), cell nuclei (DAPI, blue), and sparsely labeled neurons (EGFP, green), in sections from mouse hippocampus was followed by tissue laser branding, sample preparation for electron microscopy and serial block face EM, manual segmentation (iMOD), and rendering (Blender) of the microglia, red.
Figure 2
Figure 2
Inventory of microglia structures. Serial Block Face EM images showing segmentation of selected color-coded objects within the microglia (a–d, gold). (a) Representative micrograph containing the microglia nucleus (dark gray), where also mitochondria (orange), Golgi apparatus (indigo), and centrioles (gray) are segmented (left). 3D surface rendering of peri-nuclear objects together with high magnification inset (right). (b–d) Representative series of consecutive micrographs showing the microglia: (b) multi-vesicular bodies (clear blue) and phago-lysosomes (purple); (c) self-engulfment of a branch originating from the same microglia (turquoise); (d) early endocytic compartment (dark blue). 3D surface rendering of intra-cellular vesicles, together with high magnification inset (right). (e) Total Volume and Area of segmented structures.
See this image and copyright information in PMC

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