Complementation and hybridization evidence for additional families of type I DNA restriction and modification genes in Salmonella serotypes
- PMID: 3056913
- PMCID: PMC211683
- DOI: 10.1128/jb.170.12.5785-5788.1988
Complementation and hybridization evidence for additional families of type I DNA restriction and modification genes in Salmonella serotypes
Abstract
Of eight Salmonella, serB-linked hsd genes for the restriction and modification of DNA transferred to Escherichia coli/Salmonella hybrids, only two--those with SM and ST (S. muenchen and S. thompson, respectively) specificities--may have weakly complemented rSB- and none complemented rK-. An A-specific DNA probe failed to hybridize to HindIII-restricted fragments of each of the hybrids, but an SB (S. typhimurium)-specific probe hybridized to DNA from the hybrid with ST specificity. These results indicate that additional families of the type I hsd genes may exist.
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