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. 1988 Nov 25;115(1):31-7.
doi: 10.1016/0022-1759(88)90306-7.

A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells

C Czerkinsky  1 , Z Moldoveanu, J Mestecky, L A Nilsson, O Ouchterlony
Affiliations

A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells

C Czerkinsky et al. J Immunol Methods. .

Abstract

A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.

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