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. 2018 Dec 21;19(1):28.
doi: 10.3390/s19010028.

Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Pei-Heng Lin  1   2 , Sheng-Cih Huang  3 , Kuang-Po Chen  4 , Bor-Ran Li  5   6 , Yaw-Kuen Li  7   8
Affiliations

Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization

Pei-Heng Lin et al. Sensors (Basel). .

Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA⁻containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm²) with a strong preference being exhibited for shorter aniline-based linkers.

Keywords: boronic acid; electrodeposition; orientated antibody immobilization; quartz crystal microbalance.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Derivatives of the BA spacer: aniline-based BA modified through electrodeposition and the thiol-based BA modified through SAM on a QCM chip.
Figure 2
Figure 2
CV of an Au electrode of (a,b) bare (grey), thiol-based (red) BAs and aniline-based BA derivatives were in 10 mM ferricyanide. Electrical Impedance of thiol-based (c) (red) and aniline-based (d) (blue) BA derivatives were fitted by the Randles equivalent where experiment result showed in dot and the fitted curve in line.
Figure 3
Figure 3
Recorded frequency responses to 25 µg/mL Ab of (a) a thiol-based BA spacer (red lines) and (b) an aniline-based BA spacer (blue lines) modified by a QCM chip in a 1× PBS buffer under real-time surveillance. (c) Comparison of the length-dependent effects on Ab-immobilization efficiency between the electrodeposition (blue) and SAM (red) methods.
Figure 4
Figure 4
Derivatives of BA spacers: aniline-based BA modified through electrodeposition and thiol-based BA modified through SAM on a QCM chip.
See this image and copyright information in PMC

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