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. 2017 Oct 28;36(4):358-365.
doi: 10.1515/jomb-2017-0019. eCollection 2017 Oct.

Alterations of HDL Particles in Children with End-stage Renal Disease

Aleksandra Stefanović  1 , Danijela Ristovski-Kornic  2 , Jelena Kotur-Stevuljević  1 , Vesna Spasojević-Kalimanovska  1 , Jelena Vekić  1 , Milica Miljković  1 , Dusan Paripović  3 , Amira Peco-Antic  3   4 , Zorana Jelić-Ivanović  1 , Aleksandra Zeljković  1
Affiliations

Alterations of HDL Particles in Children with End-stage Renal Disease

Aleksandra Stefanović et al. J Med Biochem. .

Abstract
in English, Serbian

Background: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation.

Methods: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT).

Results: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses when compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses.

Conclusion: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality are not routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information.

Uvod: Displipidemija predstavlja jedan od osnovnih faktora rizika za razvoj kardiovaskularnih bolesti kod pacijenata sa hroničnim bubrežnim bolestima (CKD). Mijeloperoksidaza (MPO) je enzim koji dovodi do oksidativne modifikacije lipoproteinskih čestica, dok sa druge strane, paraoksonaza 1 (PON 1) predstavlja antioksidativni enzim koji sprečava ove procese. Cilj ove studije bio je ispitivanje oksidativnostresnog statusa i raspodele lipoproteinskih čestica kod dece sa različitim stadijumima CKD i nakon transplantacije bubrega. Takođe, u ovoj studiji je ispitivan odnos MPO/PON1 i njegova veza sa promenama distribucije HDL.

Metode: U studiji je učestvovalo 19 dece u različitim stadijumima CKD i 19 dece nakon transplantacije bubrega (PT). Parametri oksidativno-stresnog statusa i aktivnost enzima PON 1 određeni su spektrofotometrijskim metodama, dok je koncentracija MPO određena imunoesejem. Razdvajanje lipoproteinskih subfrakcija vršeno je metodom vertikalne gradijent gel elektoforeze.

Rezultati: U CKD grupi su dobijene statistički značajno više vrednosti odnosa MPO/PON1, kao i veći procenat malih HDL čestica u poređenju sa PT grupom. Takođe, uočena je statistički značajna pozitivna korelacija između MPO/PON1 odnosa i relativnog udela malih, HDL čestica kod ovih pacijenata.

Zaključak: Rezultati ove studije pokazuju da transplantacija kod dece sa hroničnim bolestima bubrega dovodi do pomeranja raspodele HDL subfrakcija ka česticama sa bolje očuvanim funkcionalnim svojstvima. Odnos MPO/PON1 može biti koristan marker funkcionalnosti HDL.

Keywords: HDL particles; chronic kidney disease; myeloperoxidase.

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Conflict of interest statement

Conflict of interest statement The authors stated that there are no conflicts of interest regarding the publication of this article.

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