Immunohistological demonstration of a substance related to neuropeptide Y and FMRFamide in the cephalic and thoracic nervous systems of the locust Locusta migratoria

Abstract

A neuropeptide related to the mammalian neuropeptide Y (NPY) is present in various neurosecretory cells (NSC) of the cephalic and thoracic nervous systems of the insect Locusta migratoria. Immunoreactive perikarya are detected in the protocerebrum, tritocerebrum, optic lobes and the suboesophageal and thoracic ganglia. They give rise to many immunoreactive processes that ramify extensively throughout the neuropiles. In the brain, prominent axon bundles tightly surround the tractus I to the corpora cardiaca. This fiber pattern suggests that the NPY-like substance may have a neuromodulator and/or neurotransmitter function. This substance may also have a neurohormonal role, since some immunoreactive tracts penetrate into neurohaemal organs via the nervi corporis cardiaci II and the thoracic median nerves. NCS containing NPY-like neuropeptide also display an FMRFamide-like immunoreactivity (except for the abdominal part of the metathoracic ganglion). NPY or FMRFamide antisera are not inactivated after preabsorption with FMRFamide or NPY, respectively. It might therefore be inferred that in locust NSC these two antisera recognize two distinct antigenic sites belonging either to a large polypeptide, or to two distinct neuropeptides.