Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec 25;19(12):3451-3455.
doi: 10.31557/APJCP.2018.19.12.3451.

Analysis of Polymorphism and Expression Profile of ASIC1 and IL-6 Genes in Patients with Gastric Cancer

Abdulkuddous Heydari-Mehrabadi  1 , Dor Mohammad Kordi Tamandani, Taybeh Baranzehi, Simin Hemati
Affiliations

Analysis of Polymorphism and Expression Profile of ASIC1 and IL-6 Genes in Patients with Gastric Cancer

Abdulkuddous Heydari-Mehrabadi et al. Asian Pac J Cancer Prev. .

Abstract

Gastric cancer is one of the most common upper gastrointestinal malignancies. Some Iranian provinces, such as in the northern and northwestern areas, are at a high risk, whereas the central and western provinces are at a medium and the southern regions at low risk. This study was carried out to estimate the impact of the expression patterns of ASIC1 and IL-6 genes and the IL-6rs-174 and ASIC1rs 75624685 polymorphisms in the pathogenesis of gastric cancer. Materials and methods: Tetra-ARMS PCR was employed to analyze the polymorphism status of the ASIC1 and IL-6 genes with 85 paraffin-embedded tissue blocks from cases and 117 normal blood samples as controls. We also investigated mRNA expression levels of these genes in 12 cases and controls using real-time PCR. Results: Our results showed a significant association between expression of ASIC1 and elevated risk of gastric cancer (p<0.001).

Keywords: Gastric cancer; polymorphism; expression; IL-6; ASIC1.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The Products of Tetra-ARMS PCR for ASIC1 (rs75624685). Control band 332bp and GG band 204bp.
Figure 2
Figure 2
The Products of Tetra-ARMS PCR for IL-6 174. Control band 326 bp and G band 204bp.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Akira S, Taga T, Kishimoto T. Interleukin-6 in biology and medicine. Adv Immunol. 1993;54:1–78. - PubMed
    1. Amjadi O, Rafiei A, Ajami A, Hosseini V, Asgarian-Omran H. Inflammation, a key factor in cancer ambush. Res Mol Med. 2014a;2:1–15.
    1. Chen L, Yuan W, Chen Z, et al. Vasoactive intestinal peptide represses activation of tumor-associated macrophages in gastric cancer via regulation of TNFα, IL-6, IL-12 and iNOS. Int J Oncol. 2015;47:1361–70. - PubMed
    1. Duch CR, Figueiredo MS, Ribas C, et al. Analysis of polymorphism at site -174 G/C of interleukin-6 promoter region in multiple myeloma. Braz J Med Biol Res. 2007;40:265–7. - PubMed
    1. Furukawa K, Takahashi T, Arai F, Matsushima K, Asakura H. Enhanced mucosal expression of interleukin-6 mRNA but not of interleukin-8 mRNA at the margin of gastric ulcer in Helicobacter pylori-positive gastritis. J Gastroenterol. 1998;33:625–33. - PubMed