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Published Erratum
. 2019 Jan 2;116(1):337.
doi: 10.1073/pnas.1819306115. Epub 2018 Dec 24.

Correction for Smith et al., Stem cell-derived clade F AAVs mediate high-efficiency homologous recombination-based genome editing

No authors listed
Published Erratum

Correction for Smith et al., Stem cell-derived clade F AAVs mediate high-efficiency homologous recombination-based genome editing

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 5.
Fig. 5.
AAVHSCs mediate efficient in vivo genome editing after i.v. injection. (A) Schema showing the vector and genome maps for the Rosa26-Luc editing vector that inserts the promoterless luciferase ORF into intron 1 of the murine Rosa26 locus. The luciferase ORF is preceded by SA/T2A (SA/2A) and followed by pA (PA). The luciferase cassette is flanked by 800-bp HAs targeting intron 1 of Rosa26. HAL, left HA; HAR, right HA. Also depicted is the map of the edited Rosa26 locus. (B) Serial in vivo imaging of luciferase expression in mice injected with AAVHSC15 Rosa26-Luc vector (5e11 vg per mouse). Luciferase expression is under the control of the chromosomal Rosa26 promoter. Also shown are AAVHSC15 noHA, a negative control containing the luciferase cassette but noHA, and an AAV8 Rosa26-Luc editing vector. Days postinjection (D) are depicted below each image. The ventral (Upper) and dorsal (Lower) images of each mouse are shown. Sample sizes for the experimental groups are as follows: AAVHSC15 Rosa26-Luc group, n = 5; AAVHSC15 noHA group, n = 3; and AAV8 Rosa26-Luc group, n = 3. (C) Sanger sequence analysis of the junction sequences between the luciferase cassette and HA, and HA and genomic DNA (gDNA) confirmed precise editing of the luciferase ORF into the Rosa26 locus. (D) Southern blot analysis of the edited murine Rosa26 locus. Genomic DNA from the liver was digested with SpeI, gel-electrophoresed, transferred to a nylon membrane, and hybridized with a luciferase-specific probe. The expected 7-kb band is observed with the luciferase probe in the edited liver DNA (lane 5), but not from untreated liver DNA (lane 4). Also shown is a titration of the vector plasmid as a positive control for the probe (lanes 1–3). MW, molecular weight marker. Maps show the location of the 7-kb band in the edited Rosa26 locus and the 3,049-bp band in the Rosa26-Luc vector plasmid (pRosa26-Luc) cut with DrdI and SpeI. Potential sizes are also depicted on a map of the vector genome. Red double-headed arrows depict the luciferase-binding site.
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