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. 2018 Dec 10;62(3):395-403.
doi: 10.2478/jvetres-2018-0056. eCollection 2018 Sep.

Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs

Ge Bai  1 , Hui Li  1 , Yansong Ge  1 , Qianzhen Zhang  1 , Jiantao Zhang  1 , Mingzi Chen  1 , Tao Liu  1 , Hongbin Wang  1
Affiliations

Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs

Ge Bai et al. J Vet Res. .

Abstract

Introduction: The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.

Material and methods: Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.

Results: Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.

Conclusion: Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.

Keywords: autophagy; hydrogen-rich saline; ischaemia-reperfusion combined resection; liver; miniature pigs.

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Conflict of interest statement

Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
Levels of ALT (A), AST (B), and AKP (C) in serum. Values are mean ±standard deviation. *Significant difference from sham group. # Significant difference of IRI + HRS group from IRI group
Fig. 2
Fig. 2
Levels of MDA (A), CAT (B), GSH (C), and SOD (D) in liver tissue. Values are mean ±standard deviation. *Significant difference from sham group. # Significant difference of IRI + HRS group from IRI group
Fig. 3
Fig. 3
Liver cells. A – sham group (10,000×); B – sham group (12,000×); C – IRI group (10,000×); D – IRI group (12,000×); E – IRI + HRS group (10,000×); F – IRI + HRS group (12,000×); N – nuclei; M – mitochondria; black arrows indicate endoplasmic reticulum and white arrows indicate the Golgi apparatus
Fig. 4
Fig. 4
Expression levels of Beclin1 (A), mTOR (B), p62 (C), ATG5 (D), and ATG12 (E) in the liver. Values are mean ±standard deviation. *Significant difference from sham group. # Significant difference of IRI + HRS group from IRI group
Fig. 5
Fig. 5
Expression levels of Beclin1 (A), LC3B (B), and p62 (C) proteins in the three groups measured by Western blot. (D) Western blot results. Values are mean ±standard deviation. * Significant difference from sham group. # Significant difference of IRI + HRS group from IRI group
Fig. 6
Fig. 6
Liver. The results of IHC of LC3B at T3h; A and B – sham group; C and D – IRI group; E and F – IRI + HRS group. The images A, C, and E were recorded at 100× magnification, and the images B, D, and F were recorded at 400× magnification
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References

    1. Baehrecke E.H.. Autophagy: dual roles in life and death? Nature Rev Mol Cell Biol. 2005;6:505. –. - PubMed
    1. Codogno P., Meijer A.J.. Autophagy and signaling: their role in cell survival and cell death. Cell Death Differ. 2005;12:1509. –. - PubMed
    1. Cursio R., Colosetti P., Codogno P., Cuervo A M, Shen H.. The role of autophagy in liver diseases: mechanisms and potential therapeutic targets. Biomed Res Int. 2015;2015:480508. - PMC - PubMed
    1. Farre J.C., Subramani S.. Peroxisome turnover by micropexophagy: an autophagy-related process. Trends Cell Biol. 2004;14:515. –. - PubMed
    1. Filomeni G., De Zio D., Cecconi F.. Oxidative stress and autophagy: the clash between damage and metabolic needs. Cell Death Differ. 2015;22:377. –. - PMC - PubMed

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