Partial purification and characterization of the human lymphocyte colony enhancing factor (LCEF)
- PMID: 305893
- PMCID: PMC1457598
Partial purification and characterization of the human lymphocyte colony enhancing factor (LCEF)
Abstract
The cloning potential of PHA-treated T cells is significantly enhanced when lymphocyte culture fluid (LCF) from mitogen-treated lymphocytes is added to the soft agar culture system. The mitogens seem to stimulate the release of a lymphocyte colony enhancing factor (LCEF) into the culture medium. A study of the physico-chemical properties of the LCEF revealed that it is a nondialyzable, heat-labile molecule which migrates in the haptoglobin (2--2) post-transferrin region in acrylamide electrophoresis. It is stable to RNase and DNase but labile to papain and trypsin. The LCEF was partially purified from the crude LCF using a sequence of techniques--ammonium sulphate precipitation, DEAE-cellulose and Bio-Gel P-150 chromatography and disc electrophoresis. The mol. wt of the purified LCEF, determined from gel filtration chromatography, was 90,000--110,000.
Similar articles
-
Identification and partial purification of human T lymphocyte colony-enhancing factor (LCEF), distinct from T cell-growth factor.Immunology. 1983 Oct;50(2):261-8. Immunology. 1983. PMID: 6604693 Free PMC article.
-
Identification and purification of human T-lymphocyte colony-enhancing factor, TLCEF: increased production by phorbol myristate acetate.Immunology. 1985 Oct;56(2):285-93. Immunology. 1985. PMID: 3876981 Free PMC article.
-
Stimulation of human T cell colony growth by a lymphocyte colony enhancement factor derived from lymphocyte subpopulations.Nat Immun Cell Growth Regul. 1990;9(3):228-36. Nat Immun Cell Growth Regul. 1990. PMID: 1973528
-
Lymphocyte chemotaxis in inflammation. VII. Isolation and purification of chemotactic factors for T lymphocytes from PPD-induced delayed hypersensitivity skin reaction site in the guinea-pig.Immunology. 1984 Feb;51(2):275-85. Immunology. 1984. PMID: 6607210 Free PMC article.
-
Partial characterization of an immunoenhancing factor from allogeneic human lymphocyte cell lines.Immunology. 1976 Jan;30(1):69-81. Immunology. 1976. PMID: 765266 Free PMC article.
Cited by
-
Activation of human lymphocytes by supernatants from human thymic epithelium.Clin Exp Immunol. 1979 Nov;38(2):348-55. Clin Exp Immunol. 1979. PMID: 160851 Free PMC article.
-
Identification and partial purification of human T lymphocyte colony-enhancing factor (LCEF), distinct from T cell-growth factor.Immunology. 1983 Oct;50(2):261-8. Immunology. 1983. PMID: 6604693 Free PMC article.
-
Identification and purification of human T-lymphocyte colony-enhancing factor, TLCEF: increased production by phorbol myristate acetate.Immunology. 1985 Oct;56(2):285-93. Immunology. 1985. PMID: 3876981 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials