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. 2018 Dec 27;6(1):146.
doi: 10.1186/s40478-018-0652-8.

A functional genomics approach to identify pathways of drug resistance in medulloblastoma

Kelsey C Bertrand  1   2 , Claudia C Faria  3 , Patryk Skowron  4   5   6   7 , Amanda Luck  4   5   6   7 , Livia Garzia  4   5   6   7 , Xiaochong Wu  4   5   6   7 , Sameer Agnihotri  8 , Christian A Smith  4   5   6   7 , Michael D Taylor  4   5   6   7 , Stephen C Mack  9   10 , James T Rutka  11   12   13   14
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A functional genomics approach to identify pathways of drug resistance in medulloblastoma

Kelsey C Bertrand et al. Acta Neuropathol Commun. .
No abstract available

Keywords: Brain tumor; Cancer; Drug resistance; Functional genomics; Medulloblastoma; Sleeping beauty; Transposon mutagenesis.

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Conflict of interest statement

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1
Fig. 1
A transposon-mutagenesis system to identify genes mediating Foretinib resistance in Medulloblastoma. a A schematic describing the steps used to treat medulloblastoma-bearing mice with Foretinib and the identification of transposon insertion sites. b A Kaplan-Meier plot demonstrating significant improvement in overall survival in mice receiving Foretinib treatment
Fig. 2
Fig. 2
Transposon insertion patterns are divergent in primary medulloblastoma receiving Foretinib therapy. a A Venn diagram illustrating the number of statistically significant gCISs exclusive or shared gCISs between vehicle (n = 14) and Foretinib (n = 12) treated primary medulloblastoma. b A table showing the Top 20 statistically significant Foretinib resistance genes in primary medulloblastoma. Highlighted in red are genes which have been reported to be mutated in cancer when compared against the COSMIC database. c Examples of transposon insertions in Cdh2 and Pten and their direction of orientation (red = anti-sense, blue = sense) relative to direction transcription (green). d Pathway analysis of Foretinib-resistance genes in primary medulloblastoma identified using GeneMania
Fig. 3
Fig. 3
Divergent patterns of transposon insertions in metastatic medulloblastoma following Foretinib therapy. a A Venn diagram illustrating the number of statistically significant gCISs identified as exclusive or shared between primary (n = 14) and metastatic medulloblastoma (n = 26). b A Venn diagram illustrating the number of statistically significant gCISs exclusive or shared gCISs between vehicle (n = 26) and Foretinib treated metastatic medulloblastoma (n = 22). c A Venn diagram comparing the gCISs between primary (n = 12) and metastatic (n = 22) Foretinib treated medulloblastoma. d A table showing the Top 20 statistically significant Foretinib resistance genes in metastatic medulloblastoma. Highlighted in red are genes, which have been reported to be mutated in cancer when compared against the COSMIC database. e Examples of transposon insertions in Basp1 and Fcgr4 and their direction of orientation (red = anti-sense, blue = sense) relative to direction transcription (green). f Pathway analysis of Foretinib-resistance genes in metastatic medulloblastoma identified using GeneMania
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References

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