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. 2018 Dec 28;293(52):20008-20009.
doi: 10.1074/jbc.H118.006869.

Structure of a transporter domain emerges

Chris Ulens  1
Affiliations

Structure of a transporter domain emerges

Chris Ulens. J Biol Chem. .

Abstract

Magnesium homeostasis relies on transporters like the CNNM family, but little information on these proteins' structure and regulation limits our understanding of their biology and functions in disease. New characterization of a conserved cytoplasmic domain now confirms the presence of a self-liganded architecture that is indispensable for Mg2+ efflux and suggests a possible role for a dimeric assembly.

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Conflict of interest statement

The author declares that he has no conflicts of interest with the contents of this article

Figures

Figure 1.
Figure 1.
Structural comparison of the HCN2 channel CNBD in complex with cAMP (PDB code 1q5o, left), the EAG1 channel CNBHD (PDB code 5k7l, middle), and the CNBHD of the CNNM3 transporter (right, this study). In the CNBHDs, the cyclic nucleotide–binding site is “self-liganded,” meaning part of the CNBHD sequence occupies the ligand binding site (Tyr-672 + Leu-674 in the case of EAG1 and Tyr-628 in the case of CNNM3). In the integral HCN and EAG channels, the CNBD quaternary assembly matches the tetramer assembly of the channel pore and C-linker domain (not shown in figure). In the CNNM3 transporter, the isolated CNBHD adopts a dimer assembly.
See this image and copyright information in PMC

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