Mediterraneibacter butyricigenes sp. nov., a butyrate-producing bacterium isolated from human faeces

Abstract

A Gram-stain-positive, obligately anaerobic, non-motile, nonspore-forming, and rod-shaped bacterial strain, designated KGMB01110T, was isolated from a faecal sample of a healthy male in South Korea. Phylogenetic analysis based on 16S rRNA gene showed that strain KGMB01110^T belonged to Clostridium cluster XIVa and was most closely related to Mediterraneibacter glycyrrhizinilyticus KCTC 5760T (95.9% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB01110^T based on its whole genome sequence was 44.1 mol%. The major cellular fatty acids (> 10%) of the isolate were C_14:0 and C_16:0. The strain KGMB01110T was positive for arginine dihydrolase, β-galactosidase-6-phosphatase, and alkaline phosphatase. The strain KGMB01110^T also produced acid from D-glucose and D-rhamnose, and hydrolyzed gelatin and aesculin. Furthermore, HPLC analysis and UV-tests of culture supernatant revealed that the strain KGMB01110^T produced butyrate as the major end product of glucose fermentation. Based on the phylogenetic and phenotypic characteristics, strain KGMB01110^T represent a novel species of the genus Mediterraneibacter in the family Lachnospiraceae. The type strain is KGMB01110^T (= KCTC 15684^T = CCUG 72830^T).

Keywords: Mediterraneibacter butyricigenes sp. nov.; butyrate; human faeces.