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. 2018 Nov 27:7:147.
doi: 10.4103/jehp.jehp_48_17. eCollection 2018.

Hepatoprotective effects of Berberis vulgaris leaf extract on carbon tetrachloride-induced hepatotoxicity in rats

Maryam Tahmasebi  1 , Heibatollah Sadeghi  2 , Habibollah Nazem  3 , Esmaiel Panahi Kokhdan  2 , Navid Omidifar  4
Affiliations

Hepatoprotective effects of Berberis vulgaris leaf extract on carbon tetrachloride-induced hepatotoxicity in rats

Maryam Tahmasebi et al. J Educ Health Promot. .

Abstract

Introduction: Hepatic sickness is a serious problem for human health. The researchers are interested in using medicinal plants including barberry to cure many of these sicknesses. In this study, the effect of hydroalcoholic extract of Berberis vulgaris leaf on hepatic protection was assessed in rats.

Materials and methods: Forty healthy male Wistar rats were divided randomly into five groups (n = 8): Group 1 (healthy control), intraperitoneal injection of olive oil; Group 2 (hepatotoxic control), intraperitoneal injection of carbon tetrachloride and daily gavage of distilled water; and testing groups, intraperitoneal injection of carbon tetrachloride along with daily gavage of B. vulgaris leaf extract 40, 80, and 120 mg/kg of weight, respectively. After 6 weeks, the following were checked: enzyme level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), as well as serum level of total protein (TP), albumin (ALB), and histopathological status of the liver.

Results: The level of AST, ALP, and ALT was increased to 109 (IU/L), 95(IU/L), and 71(IU/L), respectively, in hepatotoxic control group than healthy control group, and there was a decrease of 0.86 (g/dl) and 0.04 (g/dl) in TP and ALB levels, respectively. The B. vulgaris extract in every three doses caused a significant decrease in hepatic enzymes level. However, the TP had a significant increase in 80 and 120 mg/kg of body weight. Regarding ALB, there was no significant difference among these groups. The histopathological results were not conformed to biochemical findings.

Conclusion: Using the appropriate dose of B. vulgaris leaf extract can help the improvement of laboratory symptoms of fatty liver.

Keywords: Alanine aminotransferase; Berberis vulgaris; alkaline phosphatase; aspartate aminotransferase; carbon tetrachloride; hepatic toxicity.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Negative control. Normal structure of the liver is evident
Figure 2
Figure 2
Positive control. Steatosis is evident in the tissue
Figure 3
Figure 3
Group 1. Steatosis is evident in the tissue and the damage has not been corrected
Figure 4
Figure 4
Group 2. No definite structural abnormality is seen
Figure 5
Figure 5
Group 3. No definite structural abnormality is seen
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References

    1. Adesanoye OA, Farombi EO. Hepatoprotective effects of Vernonia amygdalina (astereaceae) in rats treated with carbon tetrachloride. Exp Toxicol Pathol. 2010;62:197–206. - PubMed
    1. Chatterjee M, Sarkar K, Sil PC. Herbal (Phyllanthus niruri) protein isolate protects liver from nimesulide induced oxidative stress. Pathophysiology. 2006;13:95–102. - PubMed
    1. Ahmed B, Alam T, Khan SA. Hepatoprotective activity of Luffa echinata fruits. J Ethnopharmacol. 2001;76:187–9. - PubMed
    1. Ramachandra Setty S, Quereshi AA, Viswanath Swamy AH, Patil T, Prakash T, Prabhu K, et al. Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia. 2007;78:451–4. - PubMed
    1. Liang D1, Zhou Q Faure P, Lafond JL. Analysis of Free Radicals in Biological Systems. Basel: Birkhauser; 1995. Measurement of plasma sulfhydryl and carbonyl groups as a possible indicator of protein oxidation.