Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1

Abstract

Light is essential for photosynthesis, but the amounts of light that exceed an organism's assimilation capacity can cause serious damage¹. Photosynthetic organisms minimize such potential harm through protection mechanisms collectively referred to as non-photochemical quenching². One mechanism of non-photochemical quenching called energy-dependent quenching (qE quenching) is readily activated under high-light conditions and dissipates excess energy as heat. LIGHT-HARVESTING COMPLEX STRESS-RELATED PROTEINS 1 and 3 (LHCSR1 and LHCSR3) have been proposed to mediate qE quenching in the green alga Chlamydomonas reinhardtii when grown under high-light conditions³. LHCSR3 induction requires a blue-light photoreceptor, PHOTOTROPIN (PHOT)⁴, although the signal transduction pathway between PHOT and LHCSR3 is not yet clear. Here, we identify two phot suppressor loci involved in qE quenching: de-etiolated 1 (det1)⁵ and damaged DNA-binding 1 (ddb1)⁶. Using a yeast two-hybrid analysis and an inhibitor assay, we determined that these two genetic elements are part of a protein complex containing CULLIN 4 (CUL4). These findings suggest a photoprotective role for the putative E3 ubiquitin ligase CUL4-DDB1^DET1 in unicellular photosynthetic organisms that may mediate blue-light signals to LHCSR1 and LHCSR3 gene expression.