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. 2018 Jul 1;37(3):355-363.
doi: 10.1515/jomb-2017-0052. eCollection 2018 Jul.

Alteration in Prooxidant-antioxidant Balance Associated with Selenium Concentration in Patients with Congenital Hypothyroidism

Shilan Rostami  1 , Asadollah Fathollahpour  2 , Mohammad Abdi  3   4 , Kejal Naderi  5
Affiliations

Alteration in Prooxidant-antioxidant Balance Associated with Selenium Concentration in Patients with Congenital Hypothyroidism

Shilan Rostami et al. J Med Biochem. .

Abstract
in English, Serbian

Background: There is a paucity of studies regarding oxidative balance and selenium (Se) status in congenital hypothyroidism. Recently, more attention has been given to the use of Se supplementation as a new treatment for thyroid disorders. Oxidative stress increases in different thyroid disorders and causes many pathological outcomes. The present study aimed to determine the serum prooxidant-anti - oxidant balance (PAB), Se, thyroid stimulating hormone (TSH) and free thyroxine (FT4) concentration in congenital hypothyroidism (CH) patients and the association of their probable change with hematological indices.

Methods: Blood samples were collected from 60 healthy and 39 CH subjects. Serum PAB values were measured and Se, TSH, FT4 and hematological indices were determined. Data were analyzed by SPSS version 16 and p value less than 0.05 was considered statistically significant.

Results: Serum TSH concentration was significantly higher in CH patients group compared to the controls (p value < 0.05); however, FT4 was in the same concentration in patients and controls. Platelet (Plt) and lymphocytes (Lym) counts markedly decreased in all patients and showed a significant direct correlation with serum TSH levels (rs = 0.307, p value = 0.004 and rs = 0.413, p value < 0.0001). Serum PAB and Se had no significant correlation with he - mato logical indices in the studied subjects.

Conclusions: Although there was no correlation between PAB and also Se with hematologic and biochemical markers in CH patients, changes in these two factors might be considered as a potential risk factor due to the other known effects of high PAB values and low Se concentrations.

Uvod: Postoji vrlo malo studija na temu oksidativnog balansa i statusa selena (Se) u kongenitalnoj hipotireozi. Odnedavno se više pažnje pridaje upotrebi suplementacije Se kao novoj terapiji za poremećaje štitne žlezde. Oksidativni stres je povišen u različitim vrstama poremećaja štitne žlezde i izaziva brojne patološke ishode. Ova studija imala je za cilj utvrđivanje prooksidantnog-antioksidantnog balansa (PAB), koncentracija Se, tireostimulišućeg hormona (TSH) i slobodnog tiroksina (FT4) u serumu kod pacijenata sa kongenitalnom hipotireozom (CH) i povezanosti njihovih verovatnih promena sa hematološkim indeksima.

Metode: Uzorci krvi su sakupljeni od 60 zdravih i 39 subjekata sa CH. Vrednosti PAB u serumu su izmerene, dok su Se, TSH, FT4 i hematološki indeksi određeni. Podaci su analizirani pomoću SPSS verzije 16 a p vrednost manja od 0,05 je smatrana statistički značajnom.

Rezultati: Koncentracija TSH u serumu bila je značajno viša u grupi pacijenata sa CH u poređenju s kontrolom (p vrednost < 0,05); međutim, FT4 bio je u istoj koncentraciji kod pacijenata i kontrolnih subjekata. Ukupni trombociti (Plt) i limfociti (Lym) bili su značajno niži kod svih pacijenata i pokazali su značajnu direktnu korelaciju s nivoima TSH userumu (r s = 0,307, p vrednost = 0,004 i r s = 0,413, p vrednost < 0,0001). PAB i Se u serumu nisu bili u značajnoj korelaciji sa hematološkim indeksima kod ispitivanih subjekata.

Zaključak: Iako nije bilo korelacije između PAB niti Se i hema toloških i biohemijskih markera kod obolelih od CH, promene ova dva faktora mogle bi se smatrati za potencijalni faktor rizika zbog drugih poznatih efekata visoke vrednosti PAB i niske koncentracije Se.

Keywords: congenital hypothyroidism; free thyroxine; prooxidant-antioxidant balance; selenium; thyroid stimulating hormone.

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Conflict of interest statement

Conflict of interest statement The authors stated that they have no conflicts of interest regarding the publication of this article.

Figures

Figure 1
Figure 1
Biochemical findings in studied groups (Mean ± SEM): 1a: Serum TSH levels (in U/mL unit) are indicated as a block value that was in a higher concentration (4.48 ± 0.69 U/mL) for the patient group and decreasing concentration to 2.7 ± 0.45 U/mL for controls with a significant p value=0.002 for their difference. 1b: According to the plot, serum FT4 has no significant differences (p value=0.067) between patients (0.089 ± 0.0083 ng/L) and controls (0.107 ± 0.0062 ng/L). 1c: The difference between serum PAB value (in HK unit) in patients (148.35 ± 7.05 HK) and controls (84.51 ± 10.51 HK) was also significant (p value=0.001). 1d: Serum Se concentration in patient subjects was lower (10.5 ± 0.65 μg/L) than in controls (55.17 ± 8.55 μg/L) and this difference was also significant (p value = 0.011).
Figure 2
Figure 2
Association between serum Se concentrations with serum PAB value: According to regression analysis, Se level (in mg/mL unit) had an indirect correlation with serum PAB value (in HK unit). The Spearman correlation showed that Se levels correlated significantly with PAB levels (r = -0.653, p value = 0.002).
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References

    1. Rastogi MV, LaFranchi SH.. Congenital hypothyroidism. Orphanet J Rare Dis. 2010;5:17. - PMC - PubMed
    1. Mirjanic-Azaric B, Avram S, Stojakovic-Jelisavac T, Sto - janovic D, Petkovic M, Bogavac-Stanojevic N, Ignjatovic S, Stojanov M. Direct estimation of reference intervals for thyroid parameters in the Republic of Srpska. J Med Biochem. 2017;36:137. –. - PMC - PubMed
    1. Hulur I, Hermanns P, Nestoris C, Heger S, Refetoff S, Pohlenz J. A single copy of the recently identified dual oxidase maturation factor (duoxa) 1 gene produces only mild transient hypothyroidism in a patient with a novel biallelic duoxa2 mutation and monoallelic duoxa1 deletion. J Clin Endocrinol Metab. 2011;96:E841. et al. –. - PMC - PubMed
    1. Jacob H, Peters C. Screening, diagnosis and management of congenital hypothyroidism: European society for paediatric endocrinology consensus guideline. Arch Dis Child Educ Pract Ed. 2015;100:260. –. - PubMed
    1. Ghasemi M, Hashemipour M, Hovsepian S, Heiydari K, Sajadi A, Hadian R. Prevalence of transient conge - nital hypothyroidism in central part of Iran. J Res Med Sci. 2013;18:699. et al. –. - PMC - PubMed