Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec;50(4):340-345.
doi: 10.3947/ic.2018.50.4.340.

Virulence Genes, Antibiotic Resistance and Capsule Locus Polymorphisms in Enterococcus faecalis isolated from Canals of Root-Filled Teeth with Periapical Lesions

Fereshteh Saffari  1 , Mohammad Hossein Sobhanipoor  2 , Arash Shahravan  3 , Roya Ahmadrajabi  4
Affiliations

Virulence Genes, Antibiotic Resistance and Capsule Locus Polymorphisms in Enterococcus faecalis isolated from Canals of Root-Filled Teeth with Periapical Lesions

Fereshteh Saffari et al. Infect Chemother. 2018 Dec.

Abstract

Frequent isolation of Enterococcus faecalis from root canal treated teeth with apical periodontitis, has proposed the role of this organism in endodontic treatment failures. Different factors have been suggested in the pathogenicity of this organism. In this study, 22 E. faecalis isolates from canals of root-filled teeth were identified, and phenotypic and genotypic characteristics were investigated. No resistance to vancomycin and gentamicin was noted, and most isolates (91%) were susceptible to ampicillin. Biofilm formation was detected in 73% of the isolates and may be considered as the most important virulence factor involved in the pathogenesis of these isolates.

Keywords: Antibiotic resistance; Apical periodontitis; Biofilm; Enterococcus faecalis; Virulence factors.

PubMed Disclaimer

Conflict of interest statement

No conflicts of interest.

Figures

Figure 1
Figure 1. Agarose gel electrophoresis PCR products of cps1, chp, ebp, ace, cps2, cyl, esp, and gelE genes (cps1: ~950 bp, chp: ~580 bp, ebp: ~101 bp, ace: ~616 bp, cps2: ~1096 bp, cyl: ~688 bp, esp: ~510 bp and gelE: ~213 bp). The products were electrophoresed on 1.5% w/v agarose gel.
PCR, polymerase chain reaction; M, 100–3,000 bp molecular size marker.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Zoletti GO, Pereira EM, Schuenck RP, Teixeira LM, Siqueira JF, Jr, dos Santos KR. Characterization of virulence factors and clonal diversity of Enterococcus faecalis isolates from treated dental root canal. Res Microbiol. 2011;162:151–158. - PubMed
    1. Fisher K, Phillips C. The ecology, epidemiology and virulence of Enterococcus. Microbiology. 2009;155:1749–1757. - PubMed
    1. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36:1–11. - PubMed
    1. Saffari F, Dalfardi MS, Mansouri S, Ahmadrajabi R. Survey for correlation between biofilm formation and virulence determinants in a collection of pathogenic and fecal Enterococcus faecalis isolates. Infect Chemother. 2017;49:176–183. - PMC - PubMed
    1. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18:268–281. - PubMed