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. 2018 Dec;50(4):340-345.
doi: 10.3947/ic.2018.50.4.340.

Virulence Genes, Antibiotic Resistance and Capsule Locus Polymorphisms in Enterococcus faecalis isolated from Canals of Root-Filled Teeth with Periapical Lesions

Fereshteh Saffari  1 , Mohammad Hossein Sobhanipoor  2 , Arash Shahravan  3 , Roya Ahmadrajabi  4
Affiliations

Virulence Genes, Antibiotic Resistance and Capsule Locus Polymorphisms in Enterococcus faecalis isolated from Canals of Root-Filled Teeth with Periapical Lesions

Fereshteh Saffari et al. Infect Chemother. 2018 Dec.

Abstract

Frequent isolation of Enterococcus faecalis from root canal treated teeth with apical periodontitis, has proposed the role of this organism in endodontic treatment failures. Different factors have been suggested in the pathogenicity of this organism. In this study, 22 E. faecalis isolates from canals of root-filled teeth were identified, and phenotypic and genotypic characteristics were investigated. No resistance to vancomycin and gentamicin was noted, and most isolates (91%) were susceptible to ampicillin. Biofilm formation was detected in 73% of the isolates and may be considered as the most important virulence factor involved in the pathogenesis of these isolates.

Keywords: Antibiotic resistance; Apical periodontitis; Biofilm; Enterococcus faecalis; Virulence factors.

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Conflict of interest statement

No conflicts of interest.

Figures

Figure 1
Figure 1. Agarose gel electrophoresis PCR products of cps1, chp, ebp, ace, cps2, cyl, esp, and gelE genes (cps1: ~950 bp, chp: ~580 bp, ebp: ~101 bp, ace: ~616 bp, cps2: ~1096 bp, cyl: ~688 bp, esp: ~510 bp and gelE: ~213 bp). The products were electrophoresed on 1.5% w/v agarose gel.
PCR, polymerase chain reaction; M, 100–3,000 bp molecular size marker.
See this image and copyright information in PMC

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