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. 2019;14(2):1559579.
doi: 10.1080/15592324.2018.1559579. Epub 2019 Jan 2.

Nitrate, but not nitrite, derived from nitrogen dioxide accumulates in Arabidopsis leaves following exposure to 15N-labeled nitrogen dioxide

Misa Takahashi  1 , Hiromichi Morikawa  1
Affiliations

Nitrate, but not nitrite, derived from nitrogen dioxide accumulates in Arabidopsis leaves following exposure to 15N-labeled nitrogen dioxide

Misa Takahashi et al. Plant Signal Behav. 2019.

Abstract

It is known that when plant leaves are exposed to exogenously applied nitrogen dioxide (NO2), nitrogen derived from NO2 is reduced to amino acid nitrogen. However, whether this is the sole metabolic fate of exogenously applied NO2 is unclear. In this study, Arabidopsis leaves were exposed to 4 ppm 15N-labeled NO2 for 4 h in light, followed by capillary ion analysis and elemental analysis-mass spectrometry with an elemental analyzer connected directly to a mass spectrometer. We found that leaf cells exposed to 15N-labeled NO2 accumulated a large amount of 15N-labeled nitrate. Neither 15N-labeled nitrite nor endogenous nitrite was present in exposed leaves. It is likely that exogenously applied NO2 is first converted to nitrite, and that nitrite is oxidized to nitrate in Arabidopsis leaf cells. The complete disappearance of nitrite derived from exogenously applied NO2 and endogenous nitrite supports this mechanism.

Keywords: nitrate; nitrogen dioxide.

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References

    1. Capron TM, Mansfield TM.. Inhibition of growth in tomato by air polluted with nitrogen oxides. J Exp Bot. 1977;28:112–116. doi: 10.1093/jxb/28.1.112 - DOI
    1. Wellburn AR. Why are atmospheric oxides of nitrogen usually phytotoxic and not alternative fertilizers?. New Phytol. 1990;115:395–429. doi: 10.1111/j.1469-8137.1990.tb00467.x - DOI - PubMed
    1. Takahashi M., Furuhashi T., Ishikawa N, Horiguchi G, Sakamoto A, Tsukaya H, Morikawa H.. Nitrogen dioxide regulates organ growth by controlling cell proliferation and enlargement in Arabidopsis. New Phytol. 2014;201:1304–1315. doi: 10.1111/nph.12609 - DOI - PubMed
    1. Takahashi M, Morikawa H.. Nitrogen dioxide is a positive regulator of plant growth. Plant Signaling Behav. 2014;9:e28033. doi: 10.4161/psb.28033 - DOI - PMC - PubMed
    1. Yoneyama T, Hideo Sasakawa H.. Transformation of atmospheric NO2 absorbed in spinach leaves. Plant Cell Physiol. 1979;20:263–266.

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