Super-resolution microscopy demystified

doi:10.1038/s41556-018-0251-8

Review

. 2019 Jan;21(1):72-84.

doi: 10.1038/s41556-018-0251-8. Epub 2019 Jan 2.

Super-resolution microscopy demystified

Lothar Schermelleh¹, Alexia Ferrand², Thomas Huser³, Christian Eggeling^{4

5}, Markus Sauer⁶, Oliver Biehlmaier², Gregor P C Drummen^{7

8}

Affiliations

¹ Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, Oxford, UK. lothar.schermelleh@bioch.ox.ac.uk.
² Imaging Core Facility, Biozentrum, University of Basel, Basel, Switzerland.
³ Biomolecular Photonics, Department of Physics, University of Bielefeld, Bielefeld, Germany.
⁴ MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
⁵ Institute for Applied Optics, Friedrich-Schiller-University Jena & Leibniz Institute of Photonic Technology, Jena, Germany.
⁶ Department of Biotechnology & Biophysics, Biocenter, Julius Maximilian University of Würzburg, Würzburg, Germany.
⁷ Advanced Bio-Imaging Program, Bio&Nano Solutions‒LAB3BIO, Bielefeld, Germany. gpcdrummen@bionano-solutions.de.
⁸ ICON-Europe.org, Exxilon Scientific Events, Steinhagen, Germany. gpcdrummen@bionano-solutions.de.

PMID: 30602772
DOI: 10.1038/s41556-018-0251-8

Review

Super-resolution microscopy demystified

Lothar Schermelleh et al. Nat Cell Biol. 2019 Jan.

. 2019 Jan;21(1):72-84.

doi: 10.1038/s41556-018-0251-8. Epub 2019 Jan 2.

Authors

Lothar Schermelleh¹, Alexia Ferrand², Thomas Huser³, Christian Eggeling^{4

5}, Markus Sauer⁶, Oliver Biehlmaier², Gregor P C Drummen^{7

8}

Affiliations

¹ Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, Oxford, UK. lothar.schermelleh@bioch.ox.ac.uk.
² Imaging Core Facility, Biozentrum, University of Basel, Basel, Switzerland.
³ Biomolecular Photonics, Department of Physics, University of Bielefeld, Bielefeld, Germany.
⁴ MRC Human Immunology Unit and Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
⁵ Institute for Applied Optics, Friedrich-Schiller-University Jena & Leibniz Institute of Photonic Technology, Jena, Germany.
⁶ Department of Biotechnology & Biophysics, Biocenter, Julius Maximilian University of Würzburg, Würzburg, Germany.
⁷ Advanced Bio-Imaging Program, Bio&Nano Solutions‒LAB3BIO, Bielefeld, Germany. gpcdrummen@bionano-solutions.de.
⁸ ICON-Europe.org, Exxilon Scientific Events, Steinhagen, Germany. gpcdrummen@bionano-solutions.de.

PMID: 30602772
DOI: 10.1038/s41556-018-0251-8

Abstract

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

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References

1. Pawley, J. B. Handbook of biological confocal microscopy. 3rd edn, (Springer US, New York, 2006). - DOI
1. Sauer, M. & Heilemann, M. Single-molecule localization microscopy in eukaryotes. Chem. Rev. 117, 7478–7509 (2017). - PubMed - DOI
1. Fornasiero, E. F. & Opazo, F. Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments. Bioessays 37, 436–451 (2015). - PubMed - DOI
1. Turkowyd, B., Virant, D. & Endesfelder, U. From single molecules to life: microscopy at the nanoscale. Anal. Bioanal. Chem. 408, 6885–6911 (2016). - PubMed - PMC - DOI
1. Eggeling, C., Willig, K. I., Sahl, S. J. & Hell, S. W. Lens-based fluorescence nanoscopy. Q. Rev. Biophys. 48, 178–243 (2015). - PubMed - DOI

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[1] Pawley, J. B. Handbook of biological confocal microscopy. 3rd edn, (Springer US, New York, 2006). - DOI

[2] Pawley, J. B. Handbook of biological confocal microscopy. 3rd edn, (Springer US, New York, 2006). - DOI

[3] Sauer, M. & Heilemann, M. Single-molecule localization microscopy in eukaryotes. Chem. Rev. 117, 7478–7509 (2017). - PubMed - DOI

[4] Sauer, M. & Heilemann, M. Single-molecule localization microscopy in eukaryotes. Chem. Rev. 117, 7478–7509 (2017). - PubMed - DOI

[5] Fornasiero, E. F. & Opazo, F. Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments. Bioessays 37, 436–451 (2015). - PubMed - DOI

[6] Fornasiero, E. F. & Opazo, F. Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments. Bioessays 37, 436–451 (2015). - PubMed - DOI

[7] Turkowyd, B., Virant, D. & Endesfelder, U. From single molecules to life: microscopy at the nanoscale. Anal. Bioanal. Chem. 408, 6885–6911 (2016). - PubMed - PMC - DOI

[8] Turkowyd, B., Virant, D. & Endesfelder, U. From single molecules to life: microscopy at the nanoscale. Anal. Bioanal. Chem. 408, 6885–6911 (2016). - PubMed - PMC - DOI

[9] Eggeling, C., Willig, K. I., Sahl, S. J. & Hell, S. W. Lens-based fluorescence nanoscopy. Q. Rev. Biophys. 48, 178–243 (2015). - PubMed - DOI

[10] Eggeling, C., Willig, K. I., Sahl, S. J. & Hell, S. W. Lens-based fluorescence nanoscopy. Q. Rev. Biophys. 48, 178–243 (2015). - PubMed - DOI

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Super-resolution microscopy demystified

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