Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jun 9;13(3):242-250.
doi: 10.1007/s13770-016-0040-z. eCollection 2016 Jun.

Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers

Guang-Zhen Jin  1   2 , Jeong-Hui Park  1   2 , Ivan Wall  1   3 , Hae-Won Kim  1   2   4
Affiliations

Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers

Guang-Zhen Jin et al. Tissue Eng Regen Med. .

Abstract

Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow. A key consideration for research is whether cell isolation methods can be improved, to reduce the process steps needed to isolate and expand cell material. In the current study, we used macroporous biopolymer microcarriers to isolate primary ADSCs. We found that the method was capable of isolating ADSCs that were subsequently capable of being transferred to culture dishes and expanded in vitro. Moreover, flow cytometry revealed that they expressed typical stem cell markers and were capable of undergoing tri-lineage differentiation. In summary, it is feasible to use biopolymer microcarriers for retrieval of viable ADSCs that retain identity markers of stem cell function.

Keywords: Adipose; Adult stem cells; Polymeric scaffolds.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7:211–228. doi: 10.1089/107632701300062859. - DOI - PubMed
    1. Zuk PA, Zhu M, Ashjian P D, Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13:4279–4295. doi: 10.1091/mbc.E02-02-0105. - DOI - PMC - PubMed
    1. Hong L, Colpan A, Peptan IA, Daw J, George A, Evans CA. 17-Beta estradiol enhances osteogenic and adipogenic differentiation of human adipose-derived stromal cells. Tissue Eng. 2007;13:1197–1203. doi: 10.1089/ten.2006.0317. - DOI - PubMed
    1. Minteer DM, Young MT, Lin YC, Over PJ, Rubin JP, Gerlach JC, et al. Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications. J Tissue Eng. 2015;6:2041731415579215. doi: 10.1177/2041731415579215. - DOI - PMC - PubMed
    1. Davies OG, Cooper PR, Shelton RM, Smith AJ, Scheven BA. Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation. J Tissue Eng. 2015;6:2041731415592356. doi: 10.1177/2041731415592356. - DOI - PMC - PubMed

LinkOut - more resources