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. 2016 Jun 9;13(3):297-303.
doi: 10.1007/s13770-016-9054-9. eCollection 2016 Jun.

Local injection of granulocyte-colony stimulating factor accelerates wound healing in a rat excisional wound model

Guang-Yin Shen  1 , In-Hwa Park  2 , Yi-Sun Song  2 , Hyun-Woo Joo  2 , Yonggu Lee  1 , Jeong-Hun Shin  1 , Kyung-Soo Kim  1   2 , Hyuck Kim  3
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Local injection of granulocyte-colony stimulating factor accelerates wound healing in a rat excisional wound model

Guang-Yin Shen et al. Tissue Eng Regen Med. .

Abstract

A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.

Keywords: Granulocyte-colony stimulating factor; Local injection; Wound healing.

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References

    1. Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med. 1999;341:738–746. doi: 10.1056/NEJM199909023411006. - DOI - PubMed
    1. Nie C, Yang D, Xu J, Si Z, Jin X, Zhang J. Locally administered adiposederived stem cells accelerate wound healing through differentiation and vasculogenesis. Cell Transplant. 2011;20:205–216. doi: 10.3727/096368910X520065. - DOI - PubMed
    1. Reddy JS, Rao PR, Reddy MS. Wound healing effects of Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in rats. J Ethnopharmacol. 2002;79:249–251. doi: 10.1016/S0378-8741(01)00388-9. - DOI - PubMed
    1. Panopoulos AD, Watowich SS. Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and ‘emergency’ hematopoiesis. Cytokine. 2008;42:277–288. doi: 10.1016/j.cyto.2008.03.002. - DOI - PMC - PubMed
    1. Minatoguchi S, Takemura G, Chen XH, Wang N, Uno Y, Koda M, et al. Acceleration of the healing process and myocardial regeneration may be important as a mechanism of improvement of cardiac function and remodeling by postinfarction granulocyte colony-stimulating factor treatment. Circulation. 2004;109:2572–2580. doi: 10.1161/01.CIR.0000129770.93985.3E. - DOI - PubMed

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