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. 2018 Dec 27;4(12):e01080.
doi: 10.1016/j.heliyon.2018.e01080. eCollection 2018 Dec.

A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences

Gloria Valentine Nakato  1   2 , Emmanuel Wicker  3   4 , Teresa A Coutinho  2 , George Mahuku  1   5 , David J Studholme  6
Affiliations

A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences

Gloria Valentine Nakato et al. Heliyon. .

Erratum in

Abstract

Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains.

Keywords: Bioinformatics; Microbiology; Molecular biology; Plant biology.

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Figures

Fig. 1
Fig. 1
a). Gel documentation results for the Avirulence protein (KFA14425.1) - Xvm specific primers on 32 Xanthomonas species to include Xanthomonas vasicola pv. musacearum (NCPPB 2005, 2251, 4378, 4379, 4380, 4381, 4383, 4384, 4386, 4387, 4388, 4389, 4390, 4391, 4392, 4393, 4394, 4395, 4433 and 4434); Xanthomonas vasicola pv. vasculorum (NCPPB (206, 795 and1381), 201500744NE, CO-5, KS-444); Xanthomonas vasicola pv. holcicola (NCPPB 989, 1241 and 2417); Xanthomonas oryzae pv. oryzae (CIX3 and 63) and Xanthomonas campestris pv. cannabis (NCPPB 2877). (b). Gel documentation results for the Avirulence protein (KFA05711.1) - Xvm specific primers on 32 Xanthomonas species to include Xanthomonas vasicola pv. musacearum (NCPPB 2005, 2251, 4378, 4379, 4380, 4381, 4383, 4384, 4386, 4387, 4388, 4389, 4390, 4391, 4392, 4393, 4394, 4395, 4433 and 4434); Xanthomonas vasicola pv. vasculorum (NCPPB (206, 795 and1381), 201500744NE, CO-5, KS-444); Xanthomonas vasicola pv. holcicola (NCPPB 989, 1241 and 2417); Xanthomonas oryzae pv. oryzae (CIX3 and 63) and Xanthomonas campestris pv. cannabis (NCPPB 2877). (c). Gel documentation results for the FIS transcriptional regulator - Xvm specific primers on 32 Xanthomonas species to include Xanthomonas vasicola pv. musacearum (NCPPB 2005, 2251, 4378, 4379, 4380, 4381, 4383, 4384, 4386, 4387, 4388, 4389, 4390, 4391, 4392, 4393, 4394, 4395, 4433 and 4434); Xanthomonas vasicola pv. vasculorum (NCPPB (206, 795 and1381), 201500744NE, CO-5, KS-444); Xanthomonas vasicola pv. holcicola (NCPPB 989, 1241 and 2417); Xanthomonas oryzae pv. oryzae (CIX3 and 63) and Xanthomonas campestris pv. cannabis (NCPPB 2877). (d). Gel documentation results for the histone-like nucleoid structuring protein - Xvm specific primers on 32 Xanthomonas species to include Xanthomonas vasicola pv. musacearum (NCPPB 2005, 2251, 4378, 4379, 4380, 4381, 4383, 4384, 4386, 4387, 4388, 4389, 4390, 4391, 4392, 4393, 4394, 4395, 4433 and 4434); Xanthomonas vasicola pv. vasculorum (NCPPB (206, 795 and1381), 201500744NE, CO-5, KS-444); Xanthomonas vasicola pv. holcicola (NCPPB 989, 1241 and 2417); Xanthomonas oryzae pv. oryzae (CIX3 and 63) and Xanthomonas campestris pv. cannabis (NCPPB 2877). (e). Gel documentation results for the Xenobiotic response element - Xvm specific primers on 32 Xanthomonas species to include Xanthomonas vasicola pv. musacearum (NCPPB 2005, 2251, 4378, 4379, 4380, 4381, 4383, 4384, 4386, 4387, 4388, 4389, 4390, 4391, 4392, 4393, 4394, 4395, 4433 and 4434); Xanthomonas vasicola pv. vasculorum (NCPPB (206, 795 and1381), 201500744NE, CO-5, KS-444); Xanthomonas vasicola pv. holcicola (NCPPB 989, 1241 and 2417); Xanthomonas oryzae pv. oryzae (CIX3 and 63) and Xanthomonas campestris pv. cannabis (NCPPB 2877).
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