doi: 10.1016/0014-5793(88)80980-3.
Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli
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- PMID: 3060375
- DOI: 10.1016/0014-5793(88)80980-3
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Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli
FEBS Lett.
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Abstract
The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.
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