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. 2019 Feb;71(1):345-350.
doi: 10.1007/s10616-018-0290-9. Epub 2019 Jan 2.

Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro

Fatih Çağlar Çelikezen  1 , Şükrü Hayta  2 , Özlem Özdemir  3 , Hasan Türkez  3   4
Affiliations

Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro

Fatih Çağlar Çelikezen et al. Cytotechnology. 2019 Feb.

Abstract

Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.

Keywords: Asteraceae; Centaurea behen L.; Cytotoxicity; Oxidative effect; Peripheral human blood.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Fig. 1
Fig. 1
The obtain MTT data in human whole blood cell cultures treated with Centaurea behen for 48 h. The results are demonstrated as mean ± SD for each group. The different letters demonstrate important variations from each other (p < 0.05)
Fig. 2
Fig. 2
The LDH release (%) values in human whole blood cell cultures induced with Centaurea behen for 48 h. The different letters indicate important differences from each other (p < 0.05)
See this image and copyright information in PMC

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