Flavobacterium sharifuzzamanii sp. nov., Isolated from the Sediments of the East China Sea

Abstract

A novel bacterial strain A7.6^T was isolated from the sediments collected near the Zhairuo Island located in the East China Sea and characterized using a polyphasic approach. Cells were Gram-stain-negative, rod-shaped, non-spore forming, non-flagellated but motile by gliding. The strain was aerobic, positive for oxidase and catalase activities. The strain can grow at 4-35 °C, pH 5.5-9.0, and 0-3% (w/v) NaCl concentration. The major polar lipid was phosphatidylethanolamine, the predominant fatty acids (> 10%) were iso-C_15:0 and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). The genomic G+C content was 33.6 mol% and the major respiratory quinone was menaquinone 6. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A7.6^T belonged to the genus Flavobacterium and was closely related to Flavobacterium tistrianum GB 56.1^T (98.4% similarity), F. nitrogenifigens NXU-44^T (98.4%), F. ginsenosidimutans THG 01^T (98.0%) and F. anhuiense D3^T (97.7%). Average nucleotide identities and digital DNA-DNA hybridizations values for genomes ranged from 75.9 to 91.4% and 21.4 to 43.9% between strain A7.6^T and its closest phylogenetic neighbors. The polyphasic characterization indicated that strain A7.6^T represented a novel species of the genus Flavobacterium, for which the name Flavobacterium sharifuzzamanii is proposed. The type strain is A7.6^T (= KCTC 62405^T = MCCC 1K03485^T). The NCBI GenBank accession number for the 16S rRNA gene of A7.6^T is MH396692, and for the genome sequence is QJGZ00000000. The digital protologue database (DPD) Taxon Number is TA00643.