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. 2018 Dec;29(4):111-115.
doi: 10.1294/jes.29.111. Epub 2018 Dec 18.

Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus

Hiroshi Bannai  1 , Manabu Nemoto  1 , Koji Tsujimura  1 , Takashi Yamanaka  1 , Hiroshi Kokado  1 , Takashi Kondo  1
Affiliations

Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus

Hiroshi Bannai et al. J Equine Sci. 2018 Dec.

Abstract

In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity.

Keywords: ELISA; EVA; sensitivity; specificity.

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References

    1. Balasuriya U.B. 2014. Equine viral arteritis. Vet. Clin. North Am. Equine Pract. 30: 543–560. - PubMed
    1. Chirnside E.D., Francis P.M., de Vries A.A., Sinclair R., Mumford J.A. 1995. Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus. J. Virol. Methods 54: 1–13. - PMC - PubMed
    1. Cho H.J., Entz S.C., Deregt D., Jordan L.T., Timoney P.J., McCollum W.H. 2000. Detection of antibodies to equine arteritis virus by a monoclonal antibody-based blocking ELISA. Can. J. Vet. Res. 64: 38–43. - PMC - PubMed
    1. Chung C., Wilson C., Timoney P., Adams E., Adams D.S., Chung J.S., Evermann J.F., Shuck K., Lee S.S., McGuire T.C. 2013. Comparison of an improved competitive enzyme-linked immunosorbent assay with the World Organization for Animal Health-prescribed serum neutralization assay for detection of antibody to equine arteritis virus. J. Vet. Diagn. Invest. 25: 182–188. - PubMed
    1. Duthie S., Mills H., Burr P. 2008. The efficacy of a commercial ELISA as an alternative to virus neutralisation test for the detection of antibodies to EAV. Equine Vet. J. 40: 182–183. - PubMed

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