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. 2019:1930:11-17.
doi: 10.1007/978-1-4939-9036-8_2.

Isolation of Human Peripheral Blood T-Lymphocytes

Atish Kizhakeyil  1 , Seow Theng Ong  2 , Mobashar Hussain Urf Turabe Fazil  2 , Madhavi Latha Somaraju Chalasani  2   3 , Praseetha Prasannan  2 , Navin Kumar Verma  4
Affiliations

Isolation of Human Peripheral Blood T-Lymphocytes

Atish Kizhakeyil et al. Methods Mol Biol. 2019.

Abstract

Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.

Keywords: Density gradient; Ficoll; PBMC; T-Lymphocytes.

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