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. 2019:1930:67-73.
doi: 10.1007/978-1-4939-9036-8_9.

GapmeR-Mediated Gene Silencing in Motile T-Cells

Mobashar Hussain Urf Turabe Fazil  1 , Seow Theng Ong  2 , Madhavi Latha Somaraju Chalasani  2   3 , Atish Kizhakeyil  2 , Navin Kumar Verma  2
Affiliations

GapmeR-Mediated Gene Silencing in Motile T-Cells

Mobashar Hussain Urf Turabe Fazil et al. Methods Mol Biol. 2019.

Abstract

Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are "hard-to-transfect" cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called "GapmeR", to knockdown specific gene(s) in human primary T-cells.

Keywords: GapmeR; Gene knockdown; RNAi; T-Lymphocytes.

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