[The evaluation of detection rate of Mycoplasma hominis and Ureaplasma spp. using cultural technique and polymerase chain reaction]

Abstract

In the clinical diagnostic laboratories of Chelyabinsk and St. Petersburg evaluation of detection rate of Mycoplasma hominis and Ureaplasma spp. was implemented using technique of polymerase chain reaction and cultural method. The reagents kits "Mycoplasma ACH-12" and "Ureaplasma ACH-12" were used to detect and determine antibiotics sensitivity of urogenital mycoplasma with determination of character of sensitivity of clinical isolates M.hominis and Ureaplasma spp. in sample of secretion of urethra and cervical channel. The results of study demonstrated that rate of detection of Ureaplasma spp. turned out significantly higher than M.hominis. The common coincidence of results in St. Petersburg between two techniques (polymerase chain reaction and cultural method) in case of detection of Ureaplasma spp. amounted to 97.5% and in case of detection of M.hominis - 93.5%.The common coincidence of the results in Chelyabinsk between two techniques (polymerase chain reaction and cultural method) in case of detection of Ureaplasma spp. amounted to 79.9% and in case of detection of M.hominis - 96.1%.