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. 2019 Jan 8;7(1):3.
doi: 10.1186/s40635-018-0220-3.

Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Julie Mouillaux  1   2 , Camille Allam  1   3 , Thomas Rimmelé  1   4 , Thomas Uberti  4 , Benjamin Delwarde  4 , Julien Textoris  1   2   4 , Guillaume Monneret  1   2   3 , Estelle Peronnet  5   6 , Fabienne Venet  1   2   3
Affiliations

Regulation of soluble CD127 protein release and corresponding transcripts expression in T lymphocytes from septic shock patients

Julie Mouillaux et al. Intensive Care Med Exp. .
No abstract available

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Conflict of interest statement

Ethics approval and consent to participate

Septic shock patients: this project was approved by our Institutional Review Board for ethics (“Comité de Protection des Personnes Sud-Est II”), which waived the need for informed consent, because this study was observational and performed on residual blood after completion of routine follow-up (#IRB 11236). This study is registered at the French Ministry of Research and Teaching (#DC-2008-509), at the Commission Nationale de l’Informatique et des Libertés and on clinicaltrials.gov (NCT02803346). Non-opposition to inclusion in the study was registered for each patient.

Healthy volunteers: peripheral blood from healthy volunteers was provided by the “Etablissement Français du Sang” from Lyon. According to the standardized procedure for blood donation, written informed consent was obtained from healthy volunteers and personal data were anonymized at time of blood donation and before blood transfer to our research lab.

Consent for publication

Not applicable.

Competing interests

JM, JT, and EP are employees of bioMérieux. BD, FV, GM, EP, and JT are co-inventors on three patent families covering IL-7 receptor biomarkers. This work was supported by Association Nationale de la Recherche et de la Technologie (JM, Convention Industrielle de Formation par la Recherche convention 2015/060).

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
sCD127 protein release from purified T cells from septic shock patients. sCD127 release was quantified in supernatants of purified T cells of septic shock patients (D1, n = 9) in comparison with healthy volunteers (HV, n = 13) after 48 h of culture without any stimulation. Data are presented as Tukey boxplots. Mann-Whitney tests were used to compare values between septic shock patients and HV. **p < 0.01, ***p < 0.001. See Additional file 1 for details
Fig. 2
Fig. 2
IL7R mRNA transcripts expression in purified T cells from septic shock patients. Gene expressions of the IL7R3 and IL7R7 transcripts were measured using RT-qPCR from RNA from purified T cells from septic shock patients at D1 (n = 13), D3 (n = 21), and D7 (n = 7) in comparison with HV (n = 18). Data are presented as Tukey boxplots. Mann-Whitney tests were used to compare values between septic shock patients and HV. **p < 0.01, ***p < 0.001. See Additional file 1 for details
Fig. 3
Fig. 3
sCD127 release and transcripts expression upon ex vivo stimulation of healthy volunteers’ purified T cells. a sCD127 protein release and b IL7R3 and IL7R7 mRNA transcripts expressions were measured in purified T cells from healthy volunteers (HV) stimulated ex vivo after the indicated time of incubation with IL-7 (n = 5, 10 ng/mL), anti-CD3/CD28 antibodies coated beads (n = 5, αCD3/28, 1:2 bead to cells ratio), or not stimulated (n = 10, NS). For each HV and each condition of stimulation, samples were proceeded in biological triplicates (culture wells) and technical duplicates (for ELISA only), summarized by the mean of the values for each individual. Data are presented as means and standard error of the mean. Mann-Whitney tests were used to compare values between non-stimulated and stimulated purified T cells from HV. *p < 0.05, **p < 0.01, ***p < 0.001. See Additional file 1 for details
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