Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Dec 14:9:3063.
doi: 10.3389/fmicb.2018.03063. eCollection 2018.

Anti-inflammatory and Antioxidant Properties of Probiotic Bacterium Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 in Wistar Albino Rats

Repally Ayyanna  1 , Dasari Ankaiah  1 , Venkatesan Arul  1
Affiliations

Anti-inflammatory and Antioxidant Properties of Probiotic Bacterium Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 in Wistar Albino Rats

Repally Ayyanna et al. Front Microbiol. .

Abstract

The potent antioxidant probiotic strains Lactobacillus mucosae AN1 and Lactobacillus fermentum SNR1 were assessed for anti-inflammatory properties in carrageenan (acute) and complete Freund's adjuvant-induced inflammation (chronic) models in the present study. The two probiotic strains were administered orally along with feed to the Wistar albino male rats as whole cell as well as microencapsulated form. The following experiments were performed to evaluate the anti-inflammatory properties of probiotic strains and the results were observed that the encapsulated and unencapsulated probiotic strains have exhibited statistically significant decrease in paw thickness. Percentage of inhibition in paw thickness of microencapsulated probiotic bacteria (Group VIII), unencapsulated strains (Group IX) were revealed 85 ± 13% and 77 ± 25%, respectively. In Hematoxylin and Eosin staining, results were revealed that the probiotic strains were exhibited anti-inflammatory effects on inflammation-induced paw tissues. qRT-PCR studies revealed upregulation of anti-inflammatory cytokine genes and down-regulation pro-inflammatory cytokine genes in probiotic-treated rat paw tissues. Further, the expression of anti-inflammatory and pro-inflammatory cytokines were examined using immunohistochemistry and ELISA methods. The probiotic administered rat paw tissue in different groups have exhibited the low level of lipid peroxides formation and higher anti-oxidant activities when compared to the control and inflammation control tissues.

Keywords: anti-inflammation; antioxidant activity; histopathology; immunohisto chemistry; interleukins; microencapsulation; qRT-PCR; wistar rats.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
Microencapsulation of L. mucosae AN1 and L. fermentum SNR1.
FIGURE 2
FIGURE 2
Hematoxylin and Eosin staining of rat paw tissue sections.
FIGURE 3
FIGURE 3
(A) Immunohistochemistry of rat paw tissues. (B) Immunohistochemistry of rat paw tissues.
FIGURE 4
FIGURE 4
(A) qRT-PCR. (B) qRT-PCR.
See this image and copyright information in PMC

References

    1. Amaretti A., Di Nunzio M., Pompei A., Raimondi S., Rossi M., Bordoni A. (2013). Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl. Microbiol. Biotechnol. 97 809–817. 10.1007/s00253-012-4241-7 - DOI - PubMed
    1. Amdekar S., Dwivedi D., Roy P., Kushwah S., Singh V. (2010). Probiotics: multifarious oral vaccine against infectious traumas. FEMS Immunol. Med. Microbiol. 58 299–306. 10.1111/j.1574-695X.2009.00630.x - DOI - PubMed
    1. Amdekar S., Roy P., Singh V., Kumar A., Singh R., Sharma P. (2012). Anti-inflammatory activity of lactobacillus on carrageenan-induced paw edema in male wistar rats. Int. J. Inflam. 2012:752015. 10.1155/2012/752015 - DOI - PMC - PubMed
    1. Amdekar S., Singh V., Singh R., Sharma P., Keshav P., Kumar A. (2011). Lactobacillus casei reduces the inflammatory joint damage associated with collagen-induced arthritis (CIA) by reducing the pro-inflammatory cytokines – Lactobacillus casei: COX-2 inhibitor. J. Clin. Immunol. 31 147–154. 10.1007/s10875-010-9457-7 - DOI - PubMed
    1. Arjumand W., Seth A., Sultana S. (2011). Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NFκB. TNF-α and caspase-3 expression in wistar rats. Food Chem. Toxicol. 49 2013–2021. 10.1016/j.fct.2011.05.012 - DOI - PubMed

LinkOut - more resources