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. 2018 Dec 27:21:e00299.
doi: 10.1016/j.btre.2018.e00299. eCollection 2019 Mar.

Xylan deterioration approach: Purification and catalytic behavior optimization of a novel β-1,4-d-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29

Zainab Bibi  1 , Shah Ali Ul Qader  2 , Afsheen Aman  3 , Haneef Ur Rehman  4 , Muhammad Asif Nawaz  5 , Asad Karim  6 , Irum Us Salam  1 , Muhammad Waqas  1 , Aysha Kamran  7   8
Affiliations

Xylan deterioration approach: Purification and catalytic behavior optimization of a novel β-1,4-d-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29

Zainab Bibi et al. Biotechnol Rep (Amst). .

Abstract

The β-1,4-d-xylanohydrolase is an industry valuable catalytic protein and used to synthesize xylooligosaccharides and xylose. In the current study, β-1,4-d-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29 was partially purified up to 9.5-fold with a recovery yield of 52%. It exhibited optimal catalytic activity at pH-7.0 and 50 °C within 5 min. Almost 50% activity retained at pH-4.0 to 9.0 however, 70% activity observed within the range of 40 °C to 70 °C. The β-1,4-d-xylanohydrolase showed a significant hydrolytic pattern with 48.7 kDa molecular mass. It was found that the enzymatic activity improved up to 160% with 1.0 mM ethanol. Moreover, the activity of enzyme drastically increased up to 2.3 and 1.5 fold when incubated with Tween 80 and Triton X-100 (1.0 mM), respectively. The β-1,4-d-xylanohydrolase also retained 72% activity at -80 °C after 180 days. Such a remarkable biochemical properties of β-1,4-d-xylanohydrolase make it possible to forecast its potential use in textile and food industries.

Keywords: 3′5′ dinitrosalicylic acid (PubChem CID: 11873); Ammonium sulfate (PubChem CID: 6097028); Catalysis; Characterization; Citric acid (PubChem CID: 311); Dihydrogen potassium phosphate (PubChem CID: 516951); Dipotassium hydrogen phosphate (PubChem CID: 24450); Glycine (PubChem CID: 750); Industrial use; Purification; Sodium potassium tartrate (PubChem CID: 9357); Thermal stability; Xylan (Simson Laboratories, UK) Xylose (PubChem CID: 135191); Xylanohydrolase.

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Figures

None
Graphical abstract
Fig. 1
Fig. 1
(a) Effect of reaction time and (b) temperature on the catalytic activity of β-1,4-d-xylanohydrolase from G. stearothermophilus KIBGE-IB29 (Means ± S.E., n = 3).
Fig. 2
Fig. 2
(a) Effect of pH and (b) ionic strength of selected buffer on the catalytic activity of β-1,4-d-xylanohydrolase from G. stearothermophilus KIBGE-IB29 (Means ± S.E., n = 3).
Fig. 3
Fig. 3
Native-PAGE analysis and In-gel activity assay of β-1,4-d-xylanohydrolase. Lane M: Bovine serum albumin dimer and monomer protein marker. Lane 1: Dialyzed precipitates of enzyme. Lane 2: In-gel activity analysis of enzyme.
Fig. 4
Fig. 4
Thermal stability of β-1,4-d-xylanohydrolase from G. stearothermophilus KIBGE-IB29 (Means ± S.E., n = 3).
Fig. 5
Fig. 5
Storage stability of β-1,4-d-xylanohydrolase from G. stearothermophilus KIBGE-IB29 (Means ± S.E., n = 3).
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