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. 2018 Sep;4(3):10-14.
doi: 10.18502/cmm.4.3.170.

Inhibitory effect of vitamin C on Aspergillus parasiticus growth and aflatoxin gene expression

Maryam Akbari Dana  1 , Parivash Kordbacheh  1 , Roshanak Daei Ghazvini  1 , Maryam Moazeni  2 , Ladan Nazemi  1 , Sasan Rezaie  1   3
Affiliations

Inhibitory effect of vitamin C on Aspergillus parasiticus growth and aflatoxin gene expression

Maryam Akbari Dana et al. Curr Med Mycol. 2018 Sep.

Abstract

Background and purpose: Aflatoxin is known as one of the most important mycotoxins threatening human life. This toxin is produced by Aspergillus species, which is the common cause of agricultural product contamination. The use of organic compounds has been always considered for the inhibition of fungal growth and toxin production. Regarding this, the aim of the present study was to investigate the effect of vitamin C on the rate of fungal growth, aflR gene expression, and toxin production.

Materials and methods: For the purpose of the study, first, Aspergillus parasiticus ATCC15517 was cultured in Sabouraud dextrose agar medium containing vitamin C at concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.1 mg/ml and temperature of 28°C for 72 h. Then, the amount of aflatoxin produced in the presence of vitamin C was measured through high performance liquid chromatography. Finally, by extracting the DNA of the cultured samples, the aflR gene expression level was evaluated by means of real-time polymerase chain reaction at different concentrations of vitamin C.

Results: The results showed that mycelium deformation was started at the vitamin C concentration of 50 mg/ml, and that only fungal spores were observed at higher concentrations. The levels of total aflatoxin and its subsets, namely B1, B2, G1, and G2, in the presence of vitamin C were estimated as 5.9, 1.9, 0.2, 3.5, and 0.3 ppm, respectively. On the other hand, these values were respectively obtained as 207.5, 73.6, 4.5, 123.4, and 6 ppm in the absence of vitamin C. Measurement of the expression level of aflR genes showed that the level of gene expression decreased to 68% and up to 81% at the vitamin C concentrations of 25 and 50 mg/ml, respectively.

Conclusion: This study showed that vitamin C, as a human-compatible compound, could be considered a good agent to protect agricultural products against fungal aflatoxin.

Keywords: Aflatoxin; Aspergillus parasiticus; Gene expression; Vitamin C; aflR gene.

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Conflict of interest statement

There is no conflict of interest.

Figures

Figure 1
Figure 1
Effect of vitamin C on the growth rate of fungal suspension; 1) concentrations of 25 mg/ml, 2) concentration of 50 mg/ml, and 3) concentration of 100 mg/ml (As seen in the picture, the amount of fungal mass decreases with the enhancement of vitamin C concentration.)
Figure 2
Figure 2
Comparison of afIR gene expression at different dilutions of vitamin C
See this image and copyright information in PMC

References

    1. Whitaker TB. Detecting mycotoxins in agricultural commodities. Mol Biotechnol. 2003;23(1):61–71. - PubMed
    1. Powell KA, Renwick A, Peberdy JF. The genus Aspergillus: from taxonomy and genetics to industrial application. Berlin, Germany: Springer Science & Business Media; 2013.
    1. Hamilton PB. Interrelationships of mycotoxins with nutrition. Fed Proc. 1977;36(6):1899–902. - PubMed
    1. Carlson MP, Ensley SM. Understanding fungal (mold) toxins (mycotoxins) Cooperative extension, institute of agriculture and natural resources. Lincoln: University of Nebraska; 2003.
    1. Peraica M, Radic B, laucic A, Pavlovic M. Toxic effect of mycotoxins in human. Bull World Health Organ. 1977;77(9):754–66. - PMC - PubMed

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