Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 7;55(1):8.
doi: 10.3390/medicina55010008.

Polymorphisms of ABCG2 and SLC22A12 Genes Associated with Gout Risk in Vietnamese Population

Nguyen Thuy Duong  1   2 , Nguyen Thy Ngoc  3   4 , Nguyen Tran Minh Thang  5   6   7 , Bach Thi Hoai Phuong  8 , Nguyen Thanh Nga  9 , Nguyen Doan Tinh  10 , Do Hai Quynh  11 , Nguyen Dang Ton  9   12 , Nong Van Hai  13   14
Affiliations

Polymorphisms of ABCG2 and SLC22A12 Genes Associated with Gout Risk in Vietnamese Population

Nguyen Thuy Duong et al. Medicina (Kaunas). .

Abstract

Background and objective: Gout is a common form of inflammatory arthritis caused by the crystallization of uric acid. Previous studies have demonstrated that the genetic predisposition of gout varies in different ethnic populations. However the association study of genetic variants with gout remains unknown in the Vietnamese population. Our study aimed to assess the relationship between polymorphisms in ABCG2 and SLC22A12 and gout susceptibility in Vietnamese. Materials and methods: Genomic DNA was extracted from blood of a total of 170 patients with gout and 351 healthy controls. We genotyped single nucleotide polymorphisms (SNPs): rs72552713, rs12505410 of the ABCG2 gene and rs11231825, rs7932775 of the SLC22A12 gene using polymerase chain reaction⁻restriction fragment length polymorphism (PCR⁻RFLP) and then confirmed 10% of randomly selected subjects by Sanger sequencing. Results: Three SNPs (rs72552713 and rs12505410 and rs11231825) were in accordance with Hardy⁻Weinberg Equilibrium (HWE) (p > 0.05) while rs7932775 was not (p < 0.05). For rs72552713, CT genotype was significantly different between gout patient and control groups (p < 0.001) and the T allele was associated with an increased risk of gout (OR = 21.19; 95% CI: 3.00⁻918.96; p < 0.001). Serum uric acid and hyperuricemia differed significantly between CC and CT genotype groups (p = 0.004 and 0.008, respectively). For rs11231825, a protective effect against gout risk was identified in the presence of the C allele when compared with the T allele (OR = 0.712; 95% CI: 0.526⁻0.964 p = 0.0302). In contrast, no significant difference of allele frequencies between gout patients and controls was detected for rs12505410 (p > 0.05). However, significant differences in serum uric acid and systolic blood pressure were obtained among gout patients. Conclusion: Our results suggest that ABCG2 rs72552713 and SLC22A12 rs11231825 are likely associated with gout in the Vietnamese population in which T allele may be a risk factor for gout susceptibility.

Keywords: ABCG2; SLC22A12; Vietnamese; gout; polymorphism.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

    1. Kuo C.F., Grainge M.J., Zhang W., Doherty M. Global epidemiology of gout: Prevalence, incidence and risk factors. Nat. Rev. Rheumatol. 2015;11:649–662. doi: 10.1038/nrrheum.2015.91. - DOI - PubMed
    1. Minh Hoa T.T., Darmawan J., Chen S.L., Van Hung N., Thi Nhi C., Ngoc An T. Prevalence of the rheumatic diseases in urban Vietnam: A WHO-ILAR COPCORD study. J. Rheumatol. 2003;30:2252–2256. - PubMed
    1. Kottgen A., Albrecht E., Teumer A., Vitart V., Krumsiek J., Hundertmark C., Pistis G., Ruggiero D., O’Seaghdha C.M., Haller T., et al. Genome-wide association analyses identify 18 new loci associated with serum urate concentrations. Nat. Genet. 2013;45:145–154. doi: 10.1038/ng.2500. - DOI - PMC - PubMed
    1. Tin A., Woodward O.M., Kao W.H., Liu C.T., Lu X., Nalls M.A., Shriner D., Semmo M., Akylbekova E.L., Wyatt S.B., et al. Genome-wide association study for serum urate concentrations and gout among African Americans identifies genomic risk loci and a novel urat1 loss-of-function allele. Hum. Mol. Genet. 2011;20:4056–4068. doi: 10.1093/hmg/ddr307. - DOI - PMC - PubMed
    1. Doyle L.A., Yang W., Abruzzo L.V., Krogmann T., Gao Y., Rishi A.K., Ross D.D. A multidrug resistance transporter from human mcf-7 breast cancer cells. Proc. Natl. Acad. Sci. USA. 1998;95:15665–15670. doi: 10.1073/pnas.95.26.15665. - DOI - PMC - PubMed

MeSH terms

Substances